Il 1α

An ELISA was used to detect the release of TCIPA. The test samples were collected from the serum and lung tissue homogenate supernatants of three mice in each group. Mouse c–c motif chemokine ligand 2/monocyte chemotactic protein-1 (CCL2/MCP-1, catalog no. EK0568), mouse interleukin-1 alpha (IL-1α, catalog no. EK0391), mouse interleukin-1 beta (IL-1β, catalog no. EK0394), mouse macrophage-stimulating factor (M-CSF, catalog no. EK0445), mouse transforming growth factor-beta 1 (TGFβ1, catalog no. EK0515), mouse interferon-gamma (IFN-γ, catalog no. EK0375), mouse interleukin-4 (IL-4, catalog no. EK0405) and interleukin-10 (IL-10, catalog no. EK0417) ELISA kits were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China). Mouse macrophage migration inhibitory factor (MIF, catalog no. H598-M-96, Nanjing, China) and mouse stromal cell-derived factor 1 (SDF-1, catalog no. H398-1-M-96, Nanjing, China) were purchased from Nanjing Jiancheng Bioengineering Institute.

Dulbecco’s Modified Eagle Medium (12100046), RPMI-1640 (31800022), L-glutamine (A2916801), penicillin G (15140-122), streptomycin (15140-122), amphotericin B (15290-017), and all other cell-culture reagents were purchased from Gibco BRL Life Technologies (Grand Island, NY, USA). Concanavalin A (con A) (L7647) and dimethyl sulfoxide (DMSO) (D2650) were obtained from the Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). The Multiplex ELISA Kit For Mouse Cytokine (MEK1006, GMCSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17, MCP-1, MIP-1α, RANTES, TNFα) was obtained from Boster Biological Technology (Pleasanton, CA, USA). Certified fetal bovine serum (FBS) (04-001-1A) was obtained from Biological Industries (Kibbutz Beit Haemek, Israel).