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Anti cleaved parp asp214 d64e10

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Anti-cleaved PARP (Asp214) (D64E10) is a primary antibody that detects the cleaved form of Poly(ADP-ribose) Polymerase (PARP) enzyme. PARP is a key enzyme involved in DNA repair, and its cleavage by caspases is a hallmark of apoptosis or programmed cell death.

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Lab products found in correlation

Dinaciclib, by Selleck Chemicals (1 mentions) Anti bcl xl 54h6, by Cell Signaling Technology (1 mentions) Anti mcl 1 s19, by Santa Cruz Biotechnology (1 mentions) A 1210477, by Chemietek (1 mentions) Ct a199, by Chemietek (1 mentions) Anti bim c34c5, by Cell Signaling Technology (1 mentions) Anti phospho her2 erbb2 tyr1248, by Cell Signaling Technology (1 mentions) Anti phospho akt ser473 d9e, by Cell Signaling Technology (1 mentions) Anti phospho akt thr308 244f9, by Cell Signaling Technology (1 mentions) Anti phospho s6 ribosomal protein ser240 244 d68f8, by Cell Signaling Technology (1 mentions) Anti phospho p44 42 mapk erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Anti bak, by Cell Signaling Technology (1 mentions) S2768, by Selleck Chemicals (1 mentions) Anti gapdh 6c5, by Santa Cruz Biotechnology (1 mentions) Anti cleaved caspase 3 d175, by Cell Signaling Technology (1 mentions) Anti bax d2e11, by Cell Signaling Technology (1 mentions) Anti cd204 clone sra e5, by Cosmo Bio (1 mentions) β actin sc 47778, by Santa Cruz Biotechnology (1 mentions)

2 protocols using anti cleaved parp asp214 d64e10

1

Targeted Cancer Therapy Compounds and Antibodies

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The following drugs were purchased: Dinaciclib (SCH727965) for in vitro and in vivo studies (S2768; Selleckchem), lapatinib ditosylate (Tykerb) for in vitro and in vivo studies (M1802; Abmole), neratinib for in vivo studies (M1913; Abmole), A-1210477 (CT-A121; Chemietek), A-1331852 (22963; Cayman Chemicals), tucatinib (HY-16069; Medchem), and ABT-199 (venetoclax) (CT-A199; Chemietek). The antibodies used in this study were as follows: anti-Bak (AB-1 clone for IP) (AM03; EMD Millipore), anti-Bak (3814S; Cell Signaling), anti-Bim (C34C5) (2933S; Cell Signaling), anti–BCL-xL (54H6) (2764S; Cell Signaling), anti–Bcl-2 (D55G8) (Human Specific) (4223S; Cell Signaling), anti-cleaved PARP (Asp214) (D64E10) (5625S; Cell Signaling), anti-GAPDH (6C5) (sc-32233; Santa Cruz), anti–MCL-1 (S-19) (sc-819; Santa Cruz), anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) (4370S; Cell Signaling), anti-phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) (5364S; Cell Signaling), anti-phospho-Akt (Thr308) (244F9) (4056S; Cell Signaling), anti-phospho-Akt (Ser473) (D9E) (4060S; Cell Signaling), anti-HER2/ErbB2 (29D8) (2165S; Cell Signaling), anti-phospho-HER2/ErbB2 (Tyr1248) (2247S; Cell Signaling), anti-phospho-Rpb1 CTD (Ser 2/5) (4375S; Cell Signaling), Normal Rabbit IgG for IP (sc-2027; Santa Cruz), and Normal Mouse IgG for IP (sc-2025; Santa Cruz).
Floros K.V., Jacob S., Kurupi R., Fairchild C.K., Hu B., Puchalapalli M., E. Koblinski J., Dozmorov M.G., Boikos S.A., Scaltriti M, & Faber A.C. (2021). Targeting transcription of MCL-1 sensitizes HER2-amplified breast cancers to HER2 inhibitors. Cell Death & Disease, 12(2), 179.
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2

Protein Extraction and Western Blot Analysis

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The cellular proteins were solubilized in a Tris buffer containing 2% sodium dodecyl sulfate and 10% glycerol. The following antibodies were used for Western blotting: anti-SPP1 (AF1433; R&D Systems), anti-CD204 (clone SRA-E5; CosmoBio), anti-Bcl-2 (D17C4; Cell Signaling Technology, Danvers, MA, USA), anti-Bax (D2E11; Cell Signaling Technology), anti-cleaved caspase-3 (D175; Cell Signaling Technology), anti-cleaved PARP (Asp214; D64E10; Cell Signaling Technology), anti-Bmi1 (EPR3745(2); abcam, Cambridge, UK), and β-actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA). The detected bands were quantitatively measured using Image J [28 (link)]. The original WB can be found in the Supplementary Material File S1.
Matsubara E., Komohara Y., Esumi S., Shinchi Y., Ishizuka S., Mito R., Pan C., Yano H., Kobayashi D., Fujiwara Y., Ikeda K., Sakagami T, & Suzuki M. (2022). SPP1 Derived from Macrophages Is Associated with a Worse Clinical Course and Chemo-Resistance in Lung Adenocarcinoma. Cancers, 14(18), 4374.
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