Lysotracker probe

The green LysoTracker probe (Beyotime, China) was used to indicate the lysosome, the red RB (Beyotime, China) was used to indicate the drug and the Hoechst 33342 (Beyotime, China) was used to indicate the nucleus. The images of cells were captured by using an inverted confocal microscope (Zeiss LSM510, Germany).

Dihydroartemisinin was obtained from Ourchem (Sinopharm Chemical Reagent Co, Ltd, China). Dimethyl sulfoxide (DMSO), cisplatin, acridine orange (AO), deferoxamine (DFO), N-acetyl-L-cysteine (NAC), and ferric ammonium citrate (FAC) were purchased from Sigma (USA). CCK-8 kit was obtained from Dojindo (Japan). EdU Imaging Kit was bought from Ribobio (China). Lysosome Staining Kit was obtained from Abnova (USA). Lysotracker probe and Mito-tracker Red probe were procured from Beyotime (China). ROS Kit and JC-1 kit were acquired from Beyotime (China). AO/EB Staining Kit was obtained from Senbeijia (China). Primary antibodies for rabbit polyclonal to LC3B (Abcam, ab48394), rabbit monoclonal to Cathepsin B (Abcam,ab125067), mouse monoclonal to GAPDH (Abcam, ab8245), SQSTM1/p62 mouse mAb(Cell Signaling Technology,#8588), ATG5 rabbit mAb (Cell Signaling Technology,#12994), ATG12 rabbit mAb (Cell Signaling Technology,#4180), ATG14 rabbit mAb (Cell Signaling Technology,#96752), Erk1/2 rabbit mAb(Cell Signaling Technology,#4695), p-Erk1/2 rabbit mAb (Cell Signaling Technology,#8544), p38 MAPK rabbit mAb (Cell Signaling Technology,#14451), p-p38 rabbit mAb (Cell Signaling Technology,#4631), SAPK/JNK rabbit mAb (Cell Signaling Technology,#9252), Phospho-SAPK/JNK Rabbit mAbp-JNK (Cell Signaling Technology,#4668), were used according to the protocol.