Cells were treated with curcumin as indicated in the figure legends, trypsinized and washed in cold 1x PBS. The cells were then resuspended in 1x Annexin binding buffer (BD PharMingen, San Diego, CA), and stained with Annexin V-phycoerythrin (Annexin V-PE; BD PharMingen, San Diego, CA) and 7-AAD (BD PharMingen, San Diego, CA) for 15 min at room temperature. The stained samples were analyzed using a fluorescence-activated cell sorting caliber bench-top flow cytometer (BD Biosciences, San Jose, CA). Data were analyzed for the apoptotic population using FlowJo software (Tree Star, Ashland, OR).
Annexin 5 phycoerythrin annexin 5 pe
Manufactured by BD
Annexin V-phycoerythrin (Annexin V-PE) is a fluorescently labeled protein used for the detection of apoptosis. Annexin V binds to phosphatidylserine, which is exposed on the surface of cells undergoing apoptosis. The phycoerythrin (PE) fluorescent label allows for the visualization and quantification of apoptotic cells using flow cytometry or fluorescence microscopy.
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Lab products found in correlation
7 aad, by BD (3 mentions)
Annexin 5 binding buffer, by BD (2 mentions)
Annexin binding buffer, by BD (1 mentions)
Calcusyn 2, by Biosoft (1 mentions)
Phosphate buffered saline (pbs), by Corning (1 mentions)
Epigallocatechin gallate (egcg), by Merck Group (1 mentions)
Resveratrol, by Merck Group (1 mentions)
3 protocols using annexin 5 phycoerythrin annexin 5 pe
1
Quantifying Curcumin-Induced Apoptosis
2
Apoptosis Induction in Lung Cancer
3
Synergistic Apoptosis Induction by EGCG and Resveratrol
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MDA686TU, Tu212 and SqCCy1 cells (1.5×105 cells/6-cm plate) were treated with various concentrations of EGCG (30–200 µM; Sigma-Aldrich; Merck KGaA), resveratrol (10–70 µM; Sigma-Aldrich; Merck KGaA) and their combination (EGCG: 30–80 µM; resveratrol: 10–20 µM) for 72 h, then trypsinized and washed in cold 1X PBS (Corning, Inc.). The cells were then resuspended in 1X Annexin V binding buffer (BD Biosciences), and stained with Annexin V-phycoerythrin (Annexin V-PE; BD Biosciences) and 7-AAD (BD Biosciences) for 15 min at room temperature. The stained samples were analyzed using a fluorescence-activated cell sorting caliber bench-top flow cytometer (BD Biosciences). FlowJo_v10.6.0_CL software (Tree Star, Inc.) was used for apoptosis analysis. Total apoptosis was considered the sum of early and late stage apoptosis. Combination Index (CI) values were calculated by using CalcuSyn 2.11 software (Biosoft).
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