Dimethyl sulfoxide (DMSO) and L-rhamnose were purchased by Millipore Sigma (Burlington, MA, USA). The 8.8a-deoxyoleandolide (DEO) was kindly provided by José A. Salas and Carmen Méndez (University of Oviedo, Spain), and 6-deoxyerythronolide B (6DEB) by Barrie Wilkinson and Rachel Lill (Biotica Technology Ltd., Cambridge, UK).
Dimethylsulfoxide dmso
Manufactured by Merck Group
Sourced in United States
Dimethylsulfoxide (DMSO) is a colorless, odorless, and polar aprotic solvent. It is a versatile laboratory chemical commonly used as a cryoprotectant, solvent, and cellular permeability enhancer in various scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Merck Group (2 mentions)
Penicillin, by Merck Group (2 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
7 12 dimethylbenzanthracene dmba, by Merck Group (1 mentions)
Mtt solution, by Merck Group (1 mentions)
Microplate reader, by Agilent Technologies (1 mentions)
Spectramax m2, by Molecular Devices (1 mentions)
Chip assay kit, by Merck Group (1 mentions)
Mouse enzyme linked immunosorbent assay kit, by R&D Systems (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Flavone, by Merck Group (1 mentions)
Praziquantel, by Merck Group (1 mentions)
Heat inactivated fetal bovine serum, by Lonza (1 mentions)
Flubendazole, by Merck Group (1 mentions)
Resveratrol resv, by Santa Cruz Biotechnology (1 mentions)
Melatonin mel, by Cayman Chemical (1 mentions)
Minoxidil, by Merck Group (1 mentions)
Ranolazine, by Merck Group (1 mentions)
7 protocols using dimethylsulfoxide dmso
1
DMSO and L-rhamnose synthesis
2
Breast Cancer Cell Line Culture
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MCF-7 and T47D human breast cancer cell lines were purchased from the America Type Culture Collection (Manassas, VA, USA). DMEM (UFC-Biotech, Riyadh, Saudi Arabia) supplemented with 15% (v/v) foetal calf serum (FCS, Biowhitaker, Lonza, Belgium), 2 mM glutamine, penicillin (100 IU/mL), and streptomycin (Sigma St. Louis, MO, USA) (100 g/mL) was used to maintain the cell lines. Conditions of 37 °C, 5% CO2, and 95% relative humidity were maintained on all cell lines in the incubator. A 10 mM solution of TQ (Sigma-Aldrich, Louis, MO, USA) in 10% Dimethyl sulfoxide (DMSO) (Millipore, Molsheim, France) was used for all treatments and for preparing the requisite working concentrations with cell culture medium. In both controls, the overall DMSO concentration remained less than 0.1%. 7,12-dimethylbenzanthracene (DMBA) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Corn oil was used to prepare the stock solutions of thymoquinone.
3
MSC Proliferation on Nanofibrous Scaffolds
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The MTT assay was employed to assess the proliferation
rate of MSCs on diverse scaffolds, comparing them
to a non-scaffold control. MSCs were seeded onto
nanofibrous scaffolds positioned in a 96-well culture
plate at an initial density of 5×103 cells/cm2. The cells
underwent incubation at 37°C and 5% CO2 in basal
medium. At intervals of 1-, 3-, 5-, and 7-days postseeding,
50 μL of MTT solution (5 mg/mL in PBS, from
Sigma Aldrich, USA), was introduced to each well (n=3)
and left to incubate at 37°C for 3.5 hours. Subsequently,
the supernatant was aspirated, and 200 μL of dimethyl
sulfoxide (DMSO) from Millipore, Germany, was added.
Optical density measurements were recorded at 570 nm
using a microplate reader (BioTek Instruments, USA)
with 630 nm as the reference wavelength. The identical
procedure was executed for cells cultured in the nonscaffold
group, serving as the control (27 ).
rate of MSCs on diverse scaffolds, comparing them
to a non-scaffold control. MSCs were seeded onto
nanofibrous scaffolds positioned in a 96-well culture
plate at an initial density of 5×103 cells/cm2. The cells
underwent incubation at 37°C and 5% CO2 in basal
medium. At intervals of 1-, 3-, 5-, and 7-days postseeding,
50 μL of MTT solution (5 mg/mL in PBS, from
Sigma Aldrich, USA), was introduced to each well (n=3)
and left to incubate at 37°C for 3.5 hours. Subsequently,
the supernatant was aspirated, and 200 μL of dimethyl
sulfoxide (DMSO) from Millipore, Germany, was added.
Optical density measurements were recorded at 570 nm
using a microplate reader (BioTek Instruments, USA)
with 630 nm as the reference wavelength. The identical
procedure was executed for cells cultured in the nonscaffold
group, serving as the control (27 ).
4
MTT Assay for 6-OHDA Cytotoxicity
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Almost 5 × 103 cells were seeded in every well of 96-well plates and incubated at 33 °C with 5% CO2. After treatment with 6-OHDA, 20 μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (5 mg/ml) were added to each well, and then incubated the plate for 4 h. Removed supernatant, added 150 μl of dimethylsulfoxide (DMSO) (Millipore, Bedford, MA, USA) to each well and mixed thoroughly for 16 min. Measured the optical density (OD) at 490 nm in SpectraMax M2 (Molecular Devices, California, USA).
5
ChIP, ELISA, and MTT Assays for Transcription Factor
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ChIP assay was using a ChIP Assay Kit (Millipore, Massachusetts, USA). The quantity of DNA was analyzed by qPCR, gene-speci c primers of promoter used as Table S1.
Enzyme-linked immunosorbent assay (ELISA) Quantity of IL-1β in the supernatant of NC and shMEF2D stable BV2 cells were tested using mouse enzyme-linked immunosorbent assay kits (R&D Systems, Minnesota, USA) according to manufacturer procedures.
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay NC and shMEF2D stable BV2 cells were seeded in 96-well plates (5 × 10 3 cells/well), then treated with LPS (500 ng/ml) for 24 and 48 h. 20 μl of MTT (5 mg/ml) (Millipore) were added to each well 4 hours before the very detection time. Then, after removing supernatant, 150 μl of dimethylsulfoxide (DMSO) (Millipore) was added to each well and mixed thoroughly for 10 min. The optical density was measured at 490 nm.
Enzyme-linked immunosorbent assay (ELISA) Quantity of IL-1β in the supernatant of NC and shMEF2D stable BV2 cells were tested using mouse enzyme-linked immunosorbent assay kits (R&D Systems, Minnesota, USA) according to manufacturer procedures.
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay NC and shMEF2D stable BV2 cells were seeded in 96-well plates (5 × 10 3 cells/well), then treated with LPS (500 ng/ml) for 24 and 48 h. 20 μl of MTT (5 mg/ml) (Millipore) were added to each well 4 hours before the very detection time. Then, after removing supernatant, 150 μl of dimethylsulfoxide (DMSO) (Millipore) was added to each well and mixed thoroughly for 10 min. The optical density was measured at 490 nm.
6
Anti-Parasitic Drug Screening Protocol
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Praziquantel (PZQ), 4-phenyl-1,2,5-oxadiazole-3-carbonile-2-oxide (OXA), N-acetylcysteine (NAC), flavone (Flav), and flubendazole (FBZ) were purchased from Merck Sigma-Aldrich (Lisboa, Sigma), resveratrol (Resv) from Santa Cruz Biotechnology (Heidelberg, Germany), artesunate (AS), vandetanib (VDT), curcumin (Curc), and melatonin (Mel) from Cayman Chemical (Ann Arbor, MI, USA), and the dipeptide H-L-tryptophan-L-serine-OH (H-Trp-Ser-OH, DiPept) from Bachem (Bubendorf, Switzerland). The culture medium RPMI 1640 and supplements including penicillin (10.000 U/mL)/streptomycin (10 mg/mL) were from Merck Sigma-Aldrich and heat-inactivated fetal bovine serum (FBS) from Lonza (Basel, Switzerland). For in vitro assays, stock solutions of 2-5 mg/mL were freshly prepared in 100% dimethylsulfoxide (DMSO) (Sigma-Aldrich) and stored at 4°C. These stock solutions were diluted in fresh culture medium before its addition to the well-containing adult worms.
7
Ranolazine and Minoxidil Dose Effects
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Ranolazine was obtained from Sigma-Aldrich (Dorset, UK). Minoxidil was obtained from Alfa Aesar TM (Thermo Fisher Scientific, UK). Four concentrations of Ranolazine were used: 0.625, 1.25, 2.5 and 5 μM; three concentrations of Minoxidil were used: 2.5, 5 and 50 μM. Stock solutions of Ranolazine (2 mM) and Minoxidil (31 mM) were prepared by dissolving the drugs in DMEM and 100% dimethyl sulfoxide (DMSO), respectively (Sigma-Aldrich). The stocks were kept frozen at − 20 °C until use. Control solutions for Minoxidil and combined treatment contained the final concentration of DMSO. Fresh solutions were made at desired concentrations by dilution in DMEM and warming to 37 °C prior to each experiment. Treatments were either short‐term/acute (electrophysiology) or long‐term/48 h (electrophysiology and functional assays).
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