Superdex s 200 size exclusion column

Manufactured by GE Healthcare

Sourced in Sweden

The Superdex S-200 size exclusion column is a gel filtration chromatography column used for the separation and purification of proteins, peptides, and other biomolecules. The column is composed of cross-linked agarose beads that allow for the separation of molecules based on their size and hydrodynamic radius. This column is suitable for a variety of applications, including the analysis of protein complexes, the purification of therapeutic proteins, and the desalting of protein samples.

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Lab products found in correlation

Human il 2, by Thermo Fisher Scientific (1 mentions) Mabselect protein a column, by GE Healthcare (1 mentions) Rituximab, by Roche (1 mentions) Mabselect, by Cytiva (1 mentions) Qhp anion exchange column, by GE Healthcare (1 mentions)

Spelling variants of this product

Superdex 200 (746 citations) Superdex 200 column (565 citations) Superdex 200 size exclusion column (71 citations) S-200 size-exclusion column (3 citations) S-200 Superdex (2 citations)

2 protocols using superdex s 200 size exclusion column

Affinity Purification and Characterization of ICKs and Antibodies

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The ICKs and antibodies were affinity-purified from culture supernatants by using a MabSelect Protein A column (GE Healthcare, Sweden) and size exclusion/exchange chromatography. ICK and antibodies concentration was determined by measuring the absorbance at 280nm with a molar extinction coefficient of 1.431 and purity was analyzed in non-reducing conditions on SDS-PAGE, and on an analytical Superdex S-200 size exclusion column (GE Healthcare, Sweden).
Rituximab, a chimeric mAb that recognizes human CD20 molecule, was purchased from Roche. MOPC-173 mAb (Abcam) was used as isotype-matched control. The no-alpha mutein was produced and purified at CIM as previously described (25 (link)). The human IL-2 was purchased from Peprotech. Of note, human IL-2 (hIL-2) binds to mouse βγ and αβγ IL-2Rs (12 (link)).

Casadesús A.V., Cruz B.M., Díaz W., González M.Á., Gómez T., Fernández B., González A., Ledón N., Sosa K., Castro K., López A., Plasencia C., Ramírez Y., Teillaud J.L., Hernández C., León K, & Hernández T. (2022). Potent immunomodulatory and antitumor effect of anti-CD20-IL2no-alpha tri-functional immunocytokine for cancer therapy. Frontiers in Immunology, 13, 1021828.

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Purification and Proteolytic Activity Assay

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A 200ml Superdex S200 size exclusion column (GE Healthcare, Sunnyvale, CA) was used to purify 15 ml of supernatant using 0.05 M Tris buffered saline, pH 8. 5ug of MN-rgp120 was incubated with 1 ml of 20x concentrated pooled fractions and incubated at 37C overnight. Fractions with proteolytic activity were pooled and loaded onto a 5ml QHP anion exchange column (GE). Buffer A was 20mM NaPO₄ and buffer B was 20mM NaPO₄, 1M NaCl. 1.4ug of MN-rgp120 was incubated with 60x concentrated QHP pooled fractions at 37֯C overnight.

Li S., Yu B., Byrne G., Wright M., O’Rourke S., Mesa K, & Berman P.W. (2019). Identification and CRISPR/Cas9 Knockout of the Endogenous C1s Protease in CHO Cells Eliminates Aberrant Proteolysis of Recombinantly Expressed Proteins. Biotechnology and bioengineering, 116(9), 2130-2145.

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