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1x81 fluorescence microscope

Manufactured by Yokogawa
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The 1X81 fluorescence microscope is a laboratory instrument designed for the observation and analysis of fluorescently labeled samples. It is capable of capturing high-quality images and data through the use of specialized optics and illumination systems.

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Lab products found in correlation

Csu x1 spinning disc unit, by Yokogawa (3 mentions) A1 laser scanning confocal microscope, by Nikon (3 mentions)

3 protocols using 1x81 fluorescence microscope

1

Quantitative Imaging Analysis of Cyst Morphology

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Images were acquired on an Olympus 1X81 fluorescence microscope equipped with a CSU-X1 spinning-disc unit (YOKOGAWA) or a Nikon-A1 laser scanning confocal microscope (Nikon). Fluorescence images acquired from confocal microscopy were reconstructed in 3D using Imaris8.2 (Bitplane). Measurements of cell nucleus dimensions, cyst epithelial thickness, and cyst orientation angle were performed manually using the Measurement tool in ImageJ (NIH)39 (link). When necessary, 3D reconstructed cyst images were rotated to render a cross-section appropriate for measurements. When conducting immunofluorescence analysis on PASE, we have cross-checked HOECHST staining with other antibody staining to ensure that (1) the amniotic ectoderm contains cells that are double positive for both HOECHST and corresponding amniotic marker such as TFAP2A and GATA3; (2) the amniotic ectoderm contains HOECHST positive nuclei that are negative for pluripotency markers such as NANOG and OCT4.
Shao Y., Taniguchi K., Townshend R.F., Miki T., Gumucio D.L, & Fu J. (2017). A pluripotent stem cell-based model for post-implantation human amniotic sac development. Nature Communications, 8, 208.
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2

3D Fluorescence Microscopy Reconstruction

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Images were acquired on an Olympus 1X81 fluorescence microscope equipped with a CSU-X1 spinning-disc unit (YOKOGAWA) or a Nikon-A1 laser scanning confocal microscope (Nikon). Fluorescence images acquired from confocal microscopy were reconstructed in 3D using Imaris8.2 (Bitplane).
Shao Y., Taniguchi K., Gurdziel K., Townshend R.F., Xue X., Yong K.M., Sang J., Spence J.R., Gumucio D.L, & Fu J. (2016). Self-organized amniogenesis by human pluripotent stem cells in a biomimetic implantation-like niche. Nature materials, 16(4), 419-425.
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3

3D Fluorescence Microscopy Reconstruction

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images were acquired on an Olympus 1X81 fluorescence microscope equipped with a CSU-X1 spinning-disc unit (YOKOGAWA) or a Nikon-A1 laser scanning confocal microscope (Nikon). Fluorescence images acquired from confocal microscopy were reconstructed in 3D using Imaris8.2 (Bitplane).
Shao Y., Taniguchi K., Gurdziel K., Townshend R.F., Xue X., Yong K.M., Sang J., Spence J.R., Gumucio D.L, & Fu J. (2016). Self-organized amniogenesis by human pluripotent stem cells in a biomimetic implantation-like niche. Nature materials, 16(4), 419-425.
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