Gac elisa

In uMkhanyakude, South Africa, dried blood spots were tested for HIV antibody using an enzyme-linked immunosorbent assay (ELISA). From 2006 to 2008, Vironostika HIV Uni-Form II Ag/Ab (bioMérieux, Boxtel, The Netherlands) was used, with the GAC ELISA (Bio-Rad, Marnes-la-Coquette, France) as a confirmatory test. From 2008 to 2019, the SD HIV 1/2 ELISA (V3) was used. In Gem, Kenya, rapid HIV testing followed the national testing algorithm of the Kenyan Ministry of Health. Samples were tested with Determine (Alere, Orlando, FL, USA), with positive tests confirmed with SD Bioline HIV-1/2 3.0 in 2011 and 2012 (Standard Diagnostics, Giheung-gu, Gyonggi-do, South Korea) and First Response (Premier Medical Corporation, Nadi Daman, India) in 2016, and Uni-gold (Trinity Biotech, Bray, Wicklow, Ireland) used as a referee test. In both settings, the DSS resident identification number was recorded and used for linkage of individuals’ HIV test results and data from questionnaires.

Laboratory testing was performed according to manufacturer’s instructions and standard operating procedures in the AHRI laboratories, Durban. DBS were tested for HIV antibody using an enzyme linked immunosorbent assay (ELISA). From 2006‒2008, Vironostika HIV Uni-Form II Ag/Ab (Biomerieux) was used, with the GAC ELISA (Biorad) as a confirmatory test. From 2008–2016, the SD HIV 1/2 ELISA (V3) was used.