Legend max mouse ifn β precoated elisa kit

RAW 264.7, THP1, and mBMDM cells were seeded at a density of 2 × 10⁵ cells/well in 96-well plates (BD Biosciences) or at 5 × 10⁵ cells/well in 24-well plates (BD Biosciences) and were grown overnight. After 24 h of treatment, IFN-β secretion was measured by means of the LEGEND MAX Mouse IFN-β precoated ELISA Kit (BioLegend), IL-6 secretion was assessed by a Mouse IL-6 ELISA MAX Deluxe (BioLegend) or Mouse IL-6 Platinum ELISA (eBioscience), TNF-α production was assessed using the Mouse TNF alpha ELISA Ready-SET-Go! kit (eBioscience), and the IL-1β cytokine level was measured with the Mouse or Human IL-1β ELISA Ready-SET-Go! kit (eBioscience). hBMNCs were seeded at a density of 4 × 10⁴/well in 96-well plates (BD Biosciences) and then grown overnight. After 24 h of treatment, the secretion levels of IL-6 and TNF-α were assessed using Human IL-6 ELISA MAX Deluxe (BioLegend) and Human TNF alpha ELISA MAX Deluxe (BioLegend), respectively. The plates were then analyzed on a microplate spectrophotometer system (Molecular Devices) at the appropriate wavelength.