Primary isolated fibroblasts (2 × 103 cells per well) and mesothelial cells (1.5 × 104 cells per well) were seeded and cultivated for 24 h in a 96-well plate (0.32 cm2 growth area). The Caspase 3/7 assay (Essen Bioscience, Sartorius, Göttingen, Germany, #4704) was performed according to the manufacturer’s instructions. The generated fluorescence signals were detected by IncuCyte S3-live cell imaging Systems after 24 and 72 h. Results were normalized to controls.
Caspase 3 7 assay
Manufactured by Sartorius
Sourced in Germany
The Caspase 3/7 assay is a lab equipment product designed to measure the activity of caspase-3 and caspase-7, two key enzymes involved in the apoptosis (programmed cell death) process. The assay provides a quantitative assessment of these caspase activities, which can be used as an indicator of cellular apoptosis.
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Lab products found in correlation
2 protocols using caspase 3 7 assay
1
Apoptosis Assay of Fibroblasts and Mesothelial Cells
2
Caspase-3/7 Assay for Apoptosis
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For determining rate of apoptosis, Caspase‐3/7 assay (Essen BioScience) was used. PC‐3, LNCaP, and 22Rv1 cells were reverse transfected in 96‐well‐plates in densities of 10 000, 15 000, and 12 000 cells/well, respectively. The next day, the cells were replenished with fresh medium containing Caspase‐3/7 assay reagent in concentrations 1.25 μM for PC‐3 and 22Rv1 cells, and 0.5 μM for LNCaP cells. Red fluorescence was measured at 72 h and analyzed using adherent cell‐by‐cell analysis with the IncuCyte‐system.
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