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3 protocols using monosaccharide standards

1

Characterization of Shanxi Cortex Periplocae

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Cortex Periplocae produced in Shanxi Province of China was purchased from Zhang Zhongjing Pharmaceutical Ltd. (Zhengzhou, China) DEAE-52; Sephadex G-100; DPPH; ABTS; TCA; Vc (>99.7%); K3Fe(CN)6; papain; TFA; TPTZ; monosaccharide standards (Rha, Ara, Man, Glc, Gal, Fru, and GlcA); and Dextran Mw standards (T-10, T-40, T-100, T-500, and T-1000) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).
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2

Extraction and Characterization of E. humifusa

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E. humifusa was harvested from the Anguo traditional Chinese medicine market (Baoding, Hebei Province, China). All monosaccharide standards were purchased from Solarbio Science and Technology Co. (Beijing, China), and the dextran standard was procured from Aladin company (Guangzhou, Guangdong Province, China). Other reagents used were of analytical grade.
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3

Characterization of C. cornucopioides Polysaccharides

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The fruiting body of C. cornucopioides was collected at the Junzi mountain of Shizong in Yunnan Province, P.R. Different monosaccharide standards (L-rhamnose, D -glucose, D-mannose, D-galactose, D-arabinose, and D-xylose) and DEAE-52 column (1.6 cm × 100 cm) were provided by Solarbio Biological Technology Company (BJ, CHN). Neutral red and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) were provided by Sigma Company (St Louis, MO, USA). Phosphate buffered saline, dimethyl sulfoxide (DMSO), Dulbecco’s Modified Eagle Medium (DMEM), and fetal bovine serum (FBS) were purchased from Gibco BRL (NY, USA). All the other reagents were of analytical grade.
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