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Block gene fragments

Manufactured by Integrated DNA Technologies
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Block gene fragments is a lab equipment product that serves to physically separate and isolate specific DNA or RNA sequences from a larger genetic sample. It operates by creating physical barriers or 'blocks' that can be used to extract and purify targeted genetic material.

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Lab products found in correlation

Close spelling variants of this product

Gene fragments Gene blocks

2 protocols using block gene fragments

1

Construction of Chimeric HIV-1 Env Glycoproteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
A plasmid for expression of the JR-FL gp120/KB9 gp41 Env chimera was built by digesting pCO-JRFLgp160 with XbaI and BsrGI restriction enzymes and subcloning the ~1500-bp (~1500-bp) JR-FL gp120-coding fragment into the same sites of pCO-KB9gp160. Similarly, an expression plasmid for the KB9 gp120/JR-FL gp41 env chimera was generated by digesting pCO-JRFLgp160 with BsrGI and AflII restriction enzymes and subcloning the ~1100-bp JR-FL gp41-coding fragment into pCO-KB9gp160, using the same sites. This strategy results in an Env chimera in which 22 amino acids upstream of the gp120-gp41 cleavage site is derived from the gp41-donating isolate. KB9(JR-FL V1-V5) and KB9(JR-FL C1-C5) chimeras contain the variable and constant regions of JR-FL gp120 engrafted into the KB9 Env, respectively. Each gene was constructed by PCR assembly of two block gene fragments (Integrated DNA technology, Coralville, Iowa) of the corresponding sequence. An overlapping short sequence allowed assembly of the DNA fragments and the PCR product was cut with XbaI and BsrGI restriction enzymes and cloned into the same sites of pCO-KB9gp160. The constructs expressing the chimeric Envs were confirmed by restriction site and DNA sequence analysis.
Herschhorn A., Gu C., Espy N., Richard J., Finzi A, & Sodroski J.G. (2014). A broad HIV-1 inhibitor blocks envelope glycoprotein transitions critical for entry. Nature chemical biology, 10(10), 845-852.
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2

Construction of Chimeric HIV-1 Env Glycoproteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
A plasmid for expression of the JR-FL gp120/KB9 gp41 Env chimera was built by digesting pCO-JRFLgp160 with XbaI and BsrGI restriction enzymes and subcloning the ~1500-bp (~1500-bp) JR-FL gp120-coding fragment into the same sites of pCO-KB9gp160. Similarly, an expression plasmid for the KB9 gp120/JR-FL gp41 env chimera was generated by digesting pCO-JRFLgp160 with BsrGI and AflII restriction enzymes and subcloning the ~1100-bp JR-FL gp41-coding fragment into pCO-KB9gp160, using the same sites. This strategy results in an Env chimera in which 22 amino acids upstream of the gp120-gp41 cleavage site is derived from the gp41-donating isolate. KB9(JR-FL V1-V5) and KB9(JR-FL C1-C5) chimeras contain the variable and constant regions of JR-FL gp120 engrafted into the KB9 Env, respectively. Each gene was constructed by PCR assembly of two block gene fragments (Integrated DNA technology, Coralville, Iowa) of the corresponding sequence. An overlapping short sequence allowed assembly of the DNA fragments and the PCR product was cut with XbaI and BsrGI restriction enzymes and cloned into the same sites of pCO-KB9gp160. The constructs expressing the chimeric Envs were confirmed by restriction site and DNA sequence analysis.
Herschhorn A., Gu C., Espy N., Richard J., Finzi A, & Sodroski J.G. (2014). A broad HIV-1 inhibitor blocks envelope glycoprotein transitions critical for entry. Nature chemical biology, 10(10), 845-852.
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