Tech 1260 infinity 2

Applied from Sithisarn et al., 2021, the analysis of flavones in the O. indicum extracts was performed using Agilent Tech 1260 Infinity II with a 1260 DAD and 1260 vial sampler (Agilent, Santa Clara, CA, USA). A YMC C₁₈ column (150 mm × 4.6 mm, 3 µm particle size) from YMC (YMC, Kyoto, Japan) was used for the identification of the flavones. Gradient elution was performed with water/0.1% formic acid (solvent A) and acetonitrile (solvent B) at a constant flow rate of 800 µL/min. The composition was changed from 70% A to 10% A in 20 min. The column temperature was 40 °C, with an injection volume of 5 µL. UV detection was performed at 285 nm. A mass spectrometric analysis of the extracts from O. indicum was conducted using a mass spectrometer detector (G6125B) with a gas temperature of 325 °C, drying gas of 12.0 L/min and nebulizer pressure of 1294 Torr. The retention times, UV and mass spectra of the obtained peaks were compared with the reference standards.

Applied from Sithisarn et al., 2021, the analysis of flavones in the O. indicum extracts was performed using Agilent Tech 1260 Infinity II with a 1260 DAD and 1260 vial sampler (Agilent, Santa Clara, CA, USA). A YMC C₁₈ column (150 mm × 4.6 mm, 3 µm particle size) from YMC (YMC, Kyoto, Japan) was used for the identification of the flavones. Gradient elution was performed with water/0.1% formic acid (solvent A) and acetonitrile (solvent B) at a constant flow rate of 800 µL/min. The composition was changed from 70% A to 10% A in 20 min. The column temperature was 40 °C, with an injection volume of 5 µL. UV detection was performed at 285 nm. A mass spectrometric analysis of the extracts from O. indicum was conducted using a mass spectrometer detector (G6125B) with a gas temperature of 325 °C, drying gas of 12.0 L/min and nebulizer pressure of 1294 Torr. The retention times, UV and mass spectra of the obtained peaks were compared with the reference standards.