Anti met c12 antibody

Manufactured by Santa Cruz Biotechnology

The Anti-Met (C12) antibody is a laboratory research tool used to detect and study the Met protein, a receptor tyrosine kinase involved in various cellular processes. This antibody can be utilized in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to identify and analyze the Met protein in biological samples.

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Lab products found in correlation

Prolong gold antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions) Anti ddk antibody, by OriGene (1 mentions) Alexa fluor 647 conjugated goat anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions) Aposensor cell viability assay kit, by Abcam (1 mentions) Anti β actin, by Merck Group (1 mentions) Tgf β, by R&D Systems (1 mentions) Anti type 1 collagen antibody, by Southern Biotech (1 mentions)

Close spelling variants of this product

Anti-MET antibody MET antibody Anti-MET

2 protocols using anti met c12 antibody

Peptide-based Enzyme-Linked Immunoassay

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Peptides (M10 and scrambled control peptide) were obtained from GenScript (Piscataway, NJ); anti-Met (C12) antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and HRP-conjugated anti-Rabbit IgG antibody was purchased from Rockland (Limerick, PA). High-affinity polystyrene micro-titer plates were obtained from VWR (Radnor, PA); ultra-3, 3’, 5, 5’-tetramethylbenzidine (TMB)-substrate was obtained from Thermoscientific (Waltham, MA).

Akter T., Atanelishvili I., Noguchi A., Silver R.M, & Bogatkevich G.S. (2017). Establishment of an indirect ELISA for detection of the novel antifibrotic peptide M10. PLoS ONE, 12(11), e0188588.

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Smad2 Activation and Collagen Dynamics

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M10 and 10 amino acids scrambled peptides were obtained from GenScript (Piscataway, NJ), red fluorescent 5,6- carboxytetramethyl-rhodamine, succinimidyl ester (5,6-TAMRA)-conjugated M10 was purchased from BioSynthesis (Lewisville, Texas). Anti-type I collagen antibody was from Southern Biotechnology (Birmingham, AL), anti-Met (C12) antibody was from Santa Cruz Biotechnology (Santa Cruz, CA), anti-Smad2 and anti-phospho-Smad2 was from Cell Signaling Technology (Danvers, MA), anti-β-actin was from Sigma (St. Louis, MO). Alexa Fluor 647® conjugated goat anti-rabbit secondary antibody, Alexa Fluor 488^® Phalloidin, and ProLong® Gold anti-fade mountant with DAPI were obtained from Life Technologies (Grand Island, NY). Recombinant human Smad2 (NM_005901) with C-terminal MYC/DDK tag, Smad4 (NM_005359) with C-terminal MYC/DDK tag, and anti-DDK antibody were purchased from OriGene Technologies (Rockville, MD), TGFβ was from R&D Systems (Minneapolis, MN), bleomycin sulfate was from Hospira Inc. (Lake Forest, IL). ApoSENSOR^™ Cell Viability Assay Kit was obtained from BioVision (Milpitas, CA).

Atanelishvili I., Shirai Y., Akter T., Buckner T., Noguchi A., Silver R.M, & Bogatkevich G.S. (2015). M10, a Caspase Cleavage Product of the Hepatocyte Growth Factor Receptor, Interacts with Smad2 and Demonstrates Anti-Fibrotic Properties in Vitro and in Vivo. Translational research : the journal of laboratory and clinical medicine, 170, 99-111.

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