Mouse tcrβ h57 597

Manufactured by BioLegend

Mouse TCRβ (H57-597) is a lab equipment product manufactured by BioLegend. It is a monoclonal antibody that recognizes the T cell receptor beta chain (TCRβ) on mouse T cells.

Automatically generated - may contain errors

Lab products found in correlation

Lsr fortessa 2, by BD (2 mentions) 7 aminoactinomycin d 7 aad, by Thermo Fisher Scientific (2 mentions) Facsdiva, by BD (2 mentions) Cd19 1d3, by BD (2 mentions) B220 ra3 6b2, by BioLegend (2 mentions) Cd4 rm4 5, by BioLegend (2 mentions)

Close spelling variants of this product

H57-597 (19 citations) TCRβ (H57-597, (17 citations) APC anti-mouse TCRβ chain (clone H57-597), (2 citations)

2 protocols using mouse tcrβ h57 597

Flow Cytometric Identification of Mouse Lymphocytes

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Cells in suspension were incubated with 7-aminoactinomycin D (7-AAD, Thermo Fisher Scientific) plus antibodies against: mouse TCRβ (H57-597, BioLegend), B220 (RA3-6B2, BioLegend, BD Biosciences), CD19 (1D3, BD Biosciences), CD4 (RM4-5, BioLegend), mouse CD1d-α-GalCer tetramer (produced in house as above), Each reagent was titrated to determine the optimal dilution. All data were acquired on an LSR Fortessa II (BD Biosciences), and analysed with FACSDiva and FlowJo (BD) software. All samples were gated on lymphocytes, with exclusion of doublets and dead cells using forward and side scatter area, forward scatter area and height and viability dye parameters, respectively.

Johnson D.N., Ruan Z., Petley E.V., Devi S., Holz L.E., Uldrich A.P., Mak J.Y., Hor J.L., Mueller S.N., McCluskey J., Fairlie D.P., Darcy P.K., Beavis P.A., Heath W.R, & Godfrey D.I. (2022). Differential location of NKT and MAIT cells within lymphoid tissue. Scientific Reports, 12, 4034.

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Flow Cytometry Analysis of Immune Cell Subsets

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Cells were then incubated with 7-aminoactinomycin D (7-AAD, Thermo Fisher) plus antibodies against: mouse TCRβ (H57-597, BioLegend), B220 (RA3-6B2, BioLegend, BD BioSciences), CD19 (1D3, BD BioSciences), CD4 (RM4-5, BioLegend), mouse CD1d/α-GalCer (produced in house), Each reagent was titrated to determine the optimal dilution factor. All data were acquired on an LSR Fortessa™ II (BD), and analysed with FACSDiva and FlowJo (BD) software. All samples were gated on lymphocytes and with exclusion of doublets and dead cells using forward and side scatter area, forward scatter area and height and viability dye parameters, respectively.

Johnson D.N., Ruan Z., Petley E.V., Devi S., Holz L.E., Uldrich A.P., Mak J.Y.W., Hor J.L., Mueller S.N., McCluskey J., Fairlie D.P., Darcy P.K., Beavis P.A., Heath W.R., & Godfrey D.I. (2021). Differential Location of NKT and MAIT Cells within Lymphoid Tissue.

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