4 methoxyacetophenone

Thiosemicarbazide, acetophenone, 4-chloroacetophenone, 4-hydroxyacetophenone, 4-methylacetophenone, 4-methoxyacetophenone and 4-nitroacetophenone used for ligand synthesis were purchased from Sigma-Aldrich (USA). Solvents of analytical grade were purchased from E. Merck, and used as received without further purification. Agarose (molecular biology grade) and ethidium bromide were procured from Sigma-Aldrich (USA). Calf-thymus DNA (CT-DNA) and supercoiled pBR322 DNA were purchased from Bangalore Genei (India). Tris(hydroxymethyl)aminomethane-hydrochloride (Tris–HCl) buffer (pH, 7.3) was used for all DNA binding and cleavage studies.
The ligands 2-(1-phenylethylidene)hydrazinecarbothioamide (L¹), 2-(1-(4-tolyl) ethylidene)hydrazinecarbothioamide (L²), 2-(1-(4-methoxyphenyl)ethylidene)hydrazinecarbothioamide (L³), 2-(1-(4-hydroxyphenyl)ethylidene)hydrazinecarbothioamide (L⁴), 2-(1-(4-nitrophenyl)ethylidene)hydrazinecarbothioamide (L⁵) and 2-(1-(4-chlorophenyl)ethylidene)hydrazinecarbothioamide (L⁶) were synthesized by following the procedure as described in the literature.^{26 (link)}

Acetoin ≥95%, dimethyl carbonate 99%, p-toluenesulphonic acid monohydrate 98.5%, 4-methoxyacetophenone 99%, anhydrous anisole 99.7%, acetic anhydride ≥99%, anhydrous acetonitrile 99.8%, anhydrous cyclohexane 99.5%, anhydrous diethyl ether ≥99%, anhydrous propylene carbonate 99.7%, iron(iii) chloride 97%, anhydrous ethyl acetate 99.8%, anhydrous magnesium sulphate 99.5%, Nile red ≥98%, 4-nitroaniline ≥99%, 4-nitroanisole analytical standard, 4-nitrophenol spectrophotometric grade, 1,2-dimethylimidazole 98%, 1-bromodecane 98%, methanol 99.9%, dichloromethane 99.8%, acetone 99.9%, chloroform-d (CDCl₃, 99.8% D), chloroform 99.9%, dimethyl sulfoxide 99.9% were purchased from Sigma-Aldrich. Ames MPF 98/100 kits, 2-nitrofluorene and 4-nitroquinoline-N-oxide were purchased from Xenometrix. TA98 and TA100 were stored at −70 °C. QUANTOFIX® Peroxide 100 was purchased from Macherey-Nagel. N,N-Diethyl-4-nitroaniline was purchased from VWR.