Quant reverse transcriptase kit

Manufactured by Tiangen Biotech

Sourced in China

The Quant Reverse Transcriptase kit is a laboratory tool designed for the conversion of RNA to complementary DNA (cDNA). This kit provides the necessary enzymes and reagents to perform this reverse transcription process, which is a fundamental step in various molecular biology applications, such as gene expression analysis and next-generation sequencing.

Automatically generated - may contain errors

Lab products found in correlation

7500 real time pcr system, by Thermo Fisher Scientific (2 mentions) Superreal premix sybr green kit, by Tiangen Biotech (1 mentions) Rneasy mini kit, by Tiangen Biotech (1 mentions) Sybr green real time pcr master mix kit, by Takara Bio (1 mentions) Origin 7, by OriginLab (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions)

3 protocols using quant reverse transcriptase kit

Quantifying Bcl-xl Expression by qPCR

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was extracted using an RNeasy Mini kit, according to the manufacturer's instructions (TianGen Biotech Co., Ltd.). Total RNA (2 µl) was reverse transcribed in a 20-µl reaction system using a Quant Reverse Transcriptase kit (TianGen Biotech Co., Ltd.). qPCR was performed using a 7500 Real-Time PCR system (Thermo Fisher Scientific, Inc.) and a SuperReal PreMix (SYBR Green) kit (TianGen Biotech Co., Ltd.). Primers used for qPCR amplification were as follows: Bcl-xl, forward, 5′-CCTGAATGACCACCTAGAGCCTT-3′ and reverse, 5′-TCATGCCCGTCAGGAACCAG-3′; 18S rRNA, forward, 5′-GTAACCCGTTGAACCCCATT-3′ and reverse, 5′-CCATCC AATCGGTAGTAGCG-3′. The cycling conditions used were as follows: Pre-denaturation at 94°C for 2 min; denaturation at 94°C for 15 sec; annealing at 55°C for 20 sec; extension at 68°C for 35 sec. A total of 40 cycles were performed. The relative expression of Bcl-xl was normalized to 18S rRNA and was calculated using the 2^−ΔΔCq method (12 (link)).

ZHANG J., LIU J., LI H, & WANG J. (2016). β-catenin signaling pathway regulates cisplatin resistance in lung adenocarcinoma cells by upregulating Bcl-xl. Molecular Medicine Reports, 13(3), 2543-2551.

+ Open protocol

+ Expand

Quantifying Gene Expression in Oyster Mantle

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was extracted from the oyster mantle 3 or 6 days after injection. Extracted RNA was quantified by absorbance at 260 nm. Quantitative real-time PCR analysis was carried out using 2 μg of total RNA and a Quant Reverse Transcriptase Kit (Tiangen), as per the manufacturer’s instructions.
Real-time PCR analysis was carried out using an Mx3000P RT-PCR System (Stratagene). Target genes were normalized to ß-actin mRNA expression levels. The nucleotide sequence of each primer used for real-time PCR is shown in Table 1. PCR amplification was carried out as 95°C for 10 s, followed by 35 cycles of 95°C for 5 s and 60°C for 20 s in duplicate. SYBR Green Real-time PCR Master Mix Kit (TaKaRa) was used for the detection.
All of the real time PCR reactions were repeated in triplicate. The gene expression levels were calculated using the 2^–∆∆Ct method [25 (link)] and normalized relative to ß-actin mRNA at the same time point. The data from the experiments were analysed by ANOVA in Origin 7.0 (OriginLab Corporation).

Sun J., Xu G., Wang Z., Li Q., Cui Y., Xie L, & Zhang R. (2015). The Effect of NF-κB Signalling Pathway on Expression and Regulation of Nacrein in Pearl Oyster, Pinctada fucata. PLoS ONE, 10(7), e0131711.

+ Open protocol

+ Expand

Quantification of eIF4A1 and miR-133a Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

For eukaryotic translation initiation factor 4A1 (eIF4A1) detection, total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's instructions. Total RNA (2 µL) was reverse transcribed in a 20-µL reaction system using a Quant Reverse Transcriptase kit (TianGen Biotech Co., Ltd, Beijing, China). Real-time quantitative polymerase chain reaction (PCR) analysis was performed using a 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). RT-primers of eIF4A1 and miR-133a mRNAs were designed and synthesized by GenePharma (Shanghai, China) as follows: eIF4A1, 5′-AAG GCG TCA TCG AGA GTA ACT-3′ (forward) and 5′-ATG TGG CCG TTT TCC CAG TC-3′ (reverse); glyceraldehyde 3-phosphate dehydrogenase (GAPDH), 5′-AGC CTT CTC CAT GGT GGT GAA-3′ (forward) and 5′-ATC ACC ATC TTC CAG GAG CGA-3′ (reverse); miR-133a, 5′-ATA AGA ATG CGG CCG CAT TCC AAA CTA GCA GCA CTA-3′ (forward) and 5′-AGC TTT GTT TAA ACT TAA CCA TTC TAG CTT TTC C-3′ (reverse); and U6, 5′-CTC GCT TCG GCA GCA CA-3′ (forward) and 5′-AAC GCT TCA CGA ATT TGC GT-3′. The relative quantification of eIF4A1 mRNA was normalized using GAPDH, and the relative quantification of miR-133a was normalized using U6. The PCR program was set as follows: 95°C for 10 min, followed by 35 cycles of 95°C for 15 s, 60°C for 30 s, and 72°C for 45 s.

Li W., Chen A., Xiong L., Chen T., Tao F., Lu Y., He Q., Zhao L., Ou R, & Xu Y. (2017). miR-133a acts as a tumor suppressor in colorectal cancer by targeting eIF4A1. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 39(5).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!