JAC1 and Biotin-JAC1 were synthesized by the laboratory-self, with the purity of > 98%. Primary antibodies information is listed in Supplementary Table 1 . ECL advance reagent, CCK-8 Kit (C0038), Nucleoplasmic protein isolation Kit (P0027), Luciferase reporter gene Kit (RG027), Protein A/G Plus-Agarose (P2055) were purchased from Beyotime (Shanghai, China). Annexin V-FITC/PI apoptosis detection Kit (A211-02), HiScript III RT SuperMix for qPCR (+gDNA wiper) (R323-01), AceQ qPCR SYBR Green Master Mix (without ROX) were obtained from Vazyme Biotech Co (Nanjing, China). CHX (761982), MG132 (C2211) were obtained from Sigma (St. Louis, MO).
Luciferase reporter gene kit
Manufactured by Beyotime
The Luciferase reporter gene kit is a tool for the detection and quantification of gene expression. It contains the luciferase gene, which is a bioluminescent reporter that emits light when activated. The kit provides the necessary components for the assay, allowing researchers to measure the activity of the luciferase gene and infer the expression levels of the target gene of interest.
Automatically generated - may contain errors
Lab products found in correlation
Cck 8 kit, by Beyotime (1 mentions)
Hiscript 3 rt supermix for qpcr gdna wiper, by Vazyme (1 mentions)
Ecl advance reagent, by Beyotime (1 mentions)
Aceq qpcr sybr green master mix without rox, by Vazyme (1 mentions)
Protein a g plus agarose, by Beyotime (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
2 protocols using luciferase reporter gene kit
1
Synthesis and Characterization of JAC1 Proteins
2
miRNA-506-3p Binding Site Luciferase Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Kcnq1ot1 and SYPL1 3'UTR sequences containing miR-506-3p binding site or mutant binding site were synthesized by Generalbio (Anhui, China) to produce Kcnq1ot1-wilde type (WT) and SYPL1-WT or Kcnq1ot1-mutant (MUT) and SYPL1-MUT. These reporter vectors were co-transfected into Cal-27 cells with miR-506-3p mimic or mimic NC through Lipofectamine 3000 (Invitrogen) for determining luciferase activities on a luciferase reporter gene kit (Beyotime) [23 (link)].
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