Test drugs were dissolved in ethanol to produce a 5 mg/mL stock solution for calibration standard preparation. The stock solution was diluted with ethanol to produce 0.15, 0.45, 0.75, 1.00, and 1.50 μg/mL of calibration standards. HPLC was performed as previously reported [41 (link)]. In brief, a 20 μL aliquot of sample supernatant was analyzed by an HPLC system consisting of an L-7100 pump, an L-7200 autosampler, an L-7300 column oven, an L-7400 UV detector (Hitachi High-Technologies, Tokyo, Japan), and a Shimadzu CR-7A computing integrator (Shimadzu, Kyoto, Japan). The system was equipped with a 4.6 × 250 mm C18 column (6.5 μm particle size, Fujifilm Wako) maintained at 40 °C. Elution was carried out isocratically with a mobile phase consisting of acetonitrile: phosphoric acid (0.01 M; 1:1, v/v) at a flow rate of 1 mL/min. The eluate was monitored at a wavelength of 254 nm. Blank data were obtained from the blood of the control fish, as described above. Drug concentrations were determined by measuring the peak area and comparing it with the peak area of known standards. Interventional studies involving animals or humans and other studies require ethical approval, and must list the authority that provided approval and the corresponding ethical approval code.
C r7a
Manufactured by Shimadzu
Sourced in Japan
The C-R7A is a compact and versatile centrifuge from Shimadzu. It is designed for a wide range of laboratory applications, providing reliable and efficient sample preparation. The C-R7A features a high-speed motor and a range of rotor options to accommodate various sample types and volumes.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using c r7a
1
HPLC Analysis of Test Drug Concentrations
2
GC-based Sterol Quantification Protocol
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GC-2010 GC data were used for image acquisition by a data processing device (C-R7A, Shimadzu Corporation, Japan). The concentration of each marker was determined by the IS curve method. The GC measurement protocol provides 0.2 µg /mL as the lower limit of quantification. Typical GC chromatograms for a healthy subject and a patient with sitosterolemia are shown in Fig. 1
3
Serum Fatty Acid Profiling via GC
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Sera were transported to a laboratory (Clinical Pathology Laboratory, Kagoshima Co., Ltd., Kagoshima, Japan) and measured. After dispensing, the samples were derivatized by adding a
derivatizing reagent (Domestic Chemistry, Tokyo, Japan) and an internal standard solution (Domestic Chemistry), stirring and heating. Next, NaOH and n-hexane (Domestic Chemistry) were added,
shaken, and centrifuged, and the upper layer was dispensed into sample tubes. The prepared samples were measured via gas chromatography (GC-2010, Shimadzu Corp., Kyoto, Japan) using a GC
capillary column for cis-trans FAs separation (TC-70, GL Sciences, Tokyo, Japan). Concentrations of the 24-fraction FAs in total serum lipids were calculated using a data processing device
(C-R7A, Shimadzu Corp.). The following FAs were analyzed: total FAs (TFAs); palmitic acid (PA) and stearic acid (SA) as saturated FAs (SFAs); oleic acid (OA) as a monounsaturated FAs
(MUFAs); LA, dihomo-γ-LA (DGLA) and arachidonic acid (AA) as ω6 FAs; and alpha-linolenic acid (ALA), EPA, and DHA as ω3 FAs. We also calculated the EPA/AA and ω6/ω3 ratios.
derivatizing reagent (Domestic Chemistry, Tokyo, Japan) and an internal standard solution (Domestic Chemistry), stirring and heating. Next, NaOH and n-hexane (Domestic Chemistry) were added,
shaken, and centrifuged, and the upper layer was dispensed into sample tubes. The prepared samples were measured via gas chromatography (GC-2010, Shimadzu Corp., Kyoto, Japan) using a GC
capillary column for cis-trans FAs separation (TC-70, GL Sciences, Tokyo, Japan). Concentrations of the 24-fraction FAs in total serum lipids were calculated using a data processing device
(C-R7A, Shimadzu Corp.). The following FAs were analyzed: total FAs (TFAs); palmitic acid (PA) and stearic acid (SA) as saturated FAs (SFAs); oleic acid (OA) as a monounsaturated FAs
(MUFAs); LA, dihomo-γ-LA (DGLA) and arachidonic acid (AA) as ω6 FAs; and alpha-linolenic acid (ALA), EPA, and DHA as ω3 FAs. We also calculated the EPA/AA and ω6/ω3 ratios.
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