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Supersignal west pico or west dura chemiluminescent substrates

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SuperSignal West Pico or West Dura are chemiluminescent substrates used for detecting and quantifying proteins in Western blotting applications. These substrates produce a luminescent signal when catalyzed by the horseradish peroxidase (HRP) enzyme, which is commonly conjugated to secondary antibodies in Western blotting procedures.

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Lab products found in correlation

Meca 79, by BD (2 mentions) Chemidoc imaging system, by Bio-Rad (2 mentions) Image lab, by Bio-Rad (2 mentions)

Spelling variants of this product

SuperSignal West Pico Chemiluminescent Substrate (2569 citations) SuperSignal West Pico Substrate (146 citations) SuperSignal West Dura Chemiluminescent Substrate (134 citations) SuperSignal West Dura substrate (107 citations) SuperSignal West chemiluminescent substrate (27 citations) SuperSignals west Pico Chemiluminescent Substrate (6 citations) SuperSignal West Pico or Dura chemiluminescent substrates (4 citations) SuperSignal West Pico/Dura Chemiluminescent Substrate (4 citations) West Dura Chemiluminescent Substrates (4 citations) SuperSignal Dura Chemiluminescent substrate (3 citations)

2 protocols using supersignal west pico or west dura chemiluminescent substrates

1

Western Blot Analysis of PNAd

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lymph nodes were lysed in ice-cold lysis buffer, and the protein concentration of cell lysates was measured using the Bradford (Bio-Rad) assay. Equal amounts of protein were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane. The membranes were blocked with 5% non-fat milk in Tris-buffered saline (TBS)-0.05%Tween (TBST) and incubated overnight at 4°C with the following primary antibodies: MECA79 (anti-PNAd, BD Biosciences) and ERK1 (Santa Cruz Biotechnology). The blots were washed and developed with SuperSignal West Pico or West Dura chemiluminescent substrates (Thermo Scientific) using a Bio-Rad ChemiDoc imaging system, and the bands were quantified using Image Lab (Bio-Rad) software. MECA79 is a carbohydrate epitope found on a group of sulfation-decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set of antigens has been referred to as PNAd with a molecular mass of 50–250 kD. Therefore, in the western blot, bands of multiple molecular weights were detected.
Azzi J., Yin Q., Uehara M., Ohori S., Tang L., Cai K., Ichimura T., McGrath M., Maarouf O., Kefaloyianni E., Loughhead S., Petr J., Sun Q., Kwon M., Tullius S., von Andrian U.H., Cheng J, & Abdi R. (2016). Targeted Delivery of Immunomodulators to Lymph Nodes. Cell reports, 15(6), 1202-1213.
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2

Western Blot Analysis of PNAd

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lymph nodes were lysed in ice-cold lysis buffer, and the protein concentration of cell lysates was measured using the Bradford (Bio-Rad) assay. Equal amounts of protein were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane. The membranes were blocked with 5% non-fat milk in Tris-buffered saline (TBS)-0.05%Tween (TBST) and incubated overnight at 4°C with the following primary antibodies: MECA79 (anti-PNAd, BD Biosciences) and ERK1 (Santa Cruz Biotechnology). The blots were washed and developed with SuperSignal West Pico or West Dura chemiluminescent substrates (Thermo Scientific) using a Bio-Rad ChemiDoc imaging system, and the bands were quantified using Image Lab (Bio-Rad) software. MECA79 is a carbohydrate epitope found on a group of sulfation-decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set of antigens has been referred to as PNAd with a molecular mass of 50–250 kD. Therefore, in the western blot, bands of multiple molecular weights were detected.
Azzi J., Yin Q., Uehara M., Ohori S., Tang L., Cai K., Ichimura T., McGrath M., Maarouf O., Kefaloyianni E., Loughhead S., Petr J., Sun Q., Kwon M., Tullius S., von Andrian U.H., Cheng J, & Abdi R. (2016). Targeted Delivery of Immunomodulators to Lymph Nodes. Cell reports, 15(6), 1202-1213.
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