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Enterobacter cloacae

Manufactured by Leibniz Institute DSMZ
Sourced in Germany

Enterobacter cloacae is a bacterial strain maintained at the Leibniz Institute DSMZ. It is a Gram-negative, rod-shaped bacterium belonging to the Enterobacteriaceae family. The strain is available for research purposes.

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2 protocols using enterobacter cloacae

1

Microbial Consortium Cultivation Protocol

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Staphylococcus aureus (ATCC, 25923, Manassas, VA, USA), Escherichia coli (6899, DSMZ, Germany), Enterobacter cloacae (30054, DSMZ, Germany), and Pseudomonas putida (6125, DSMZ, Germany) were grown to the log phase in 15 mL Lauria broth (LB) (Himedia, M575, Thane West, India) in 50 mL tubes for about 5 h. Each culture was diluted to 0.8 ± 0.05 OD.
The artificial wastewater included 1 mL of each strain in LB (total of 4 mL) along with 10 mL yeast extract (7184A Neogen, Lexington, KY, USA), 69 mL Geobacter medium, and 17 mL phosphate buffer (PB) pH 6.8.
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2

Cultivation and Inoculation of Enterobacter cloacae

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Enterobacter cloacae (DSM 6234) was obtained from DSMZ, German Collection of Microorganisms and Cell Cultures GmbH (Germany) and was cultured in MRS modified broth96 at 30°C, with agitation, overnight. Mono-associated flies were created inoculating Enterobacter cloacae on top of the sterile diet. 300 μL of culture at an optical density of 0.7 when measuring at 600 nm (OD600) was added into 45 mm diameter vials, with a sterile Whatman filter paper.
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