HPLC grade methanol and acetonitrile, petroleum ether, CDCl3, methanol-d4, dimethyl sulfoxide, acarbose, p-nitrophenol α-D-glucopyranoside (PNPG) and α-glucosidase type I (EC 3.2.20, from Saccharomyces cerevisiae, lyophilized powder) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol and ethyl acetate used for extraction were purchased from Tedia (Fairfield, OH, USA). Formic acid was obtained from Merck (Darmstadt, Germany) and water was prepared by a deionization and 0.22-µm membrane filtration system (Milipore, Billerica, MA, USA).
P nitrophenol α d glucopyranoside pnpg
Manufactured by Merck Group
Sourced in United States
P-nitrophenol α-D-glucopyranoside (PNPG) is a synthetic substrate used in enzymatic assays. It is a colorless compound that, when cleaved by the enzyme α-glucosidase, releases p-nitrophenol, a yellow-colored product that can be measured spectrophotometrically. This property makes PNPG a useful tool for the quantitative detection and analysis of α-glucosidase activity.
Automatically generated - may contain errors
Lab products found in correlation
Methanol d4, by Merck Group (1 mentions)
Methanol and acetonitrile hplc grade, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Cdcl3, by Merck Group (1 mentions)
Petroleum ether, by Merck Group (1 mentions)
Acarbose, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Ethyl acetate, by Tedia (1 mentions)
Rutin, by Thermo Fisher Scientific (1 mentions)
Orlistat, by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
α glucosidase, by Merck Group (1 mentions)
2 protocols using p nitrophenol α d glucopyranoside pnpg
1
Enzymatic Assay for α-Glucosidase Activity
2
In vitro α-Glucosidase Inhibition Assay
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Mitragynine (Lipomed, Inc., lot number 1610.1B0.2) was kindly obtained from Assoc. Prof. Dr. Gorawit Yusakul, School of Pharmacy, Walailak University. Quercetin (Sigma-Aldrich, St Louis, MO, USA, Lot number Q4951), and rutin (Acros Organics, Lot number A0355330) α-Glucosidase (Sigma-Aldrich, St Louis, MO, USA) p-nitrophenol-α-D-glucopyranoside (pNPG) (Sigma-Aldrich, St Louis, MO, USA, Lot number 3698310) acarbose (positive control) (Sigma-Aldrich, St Louis, MO, USA, Lot number SLCF5122) orlistat (Sigma-Aldrich, St Louis, MO, USA, Lot number 0000117290).
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