Ne per nuclear cytoplasmic extraction reagents kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The NE-PER™ Nuclear Cytoplasmic Extraction Reagents Kit is a laboratory product that provides reagents for the extraction and separation of nuclear and cytoplasmic proteins from cells. The kit includes specific buffers and solutions to facilitate this process.

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Lab products found in correlation

Glutathione resin, by Takara Bio (1 mentions) Pierce crosslink ip kit, by Thermo Fisher Scientific (1 mentions) Transam nf κb transcription factor assay kit, by Active Motif (1 mentions) Bca method, by Merck Group (1 mentions)

Close spelling variants of this product

NE-PERTM Nuclear and Cytoplasmic Extraction Reagents kit NE-PER nuclear/cytoplasmic extraction kit NE-PER nuclear extraction kit NE-PER extraction kit NE-PER Nuclear Cytoplasmic/Nuclear Extraction Kit NE-PER reagents Cytoplasmic Extraction Reagent kit Cytoplasmic Extraction Reagents NE-PER kit

2 protocols using ne per nuclear cytoplasmic extraction reagents kit

GST Pull-down Assay for SerRS Domains

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Recombinant protein GST-SerRS(T429A) was purified by Glutathione resin (Clontech). GST pull-down assay of GST and GST-SerRS(T429A) with nuclear protein extracts isolated from HEK293T followed the protocol described in the NE-PER™ Nuclear Cytoplasmic Extraction Reagents Kit and Pierce™ Crosslink IP Kit (Thermo Fisher Scientific; TFS). The resultant GST pull-down products were analyzed by SDS-PAGE, followed by silver staining and in-gel digestion. For domain mapping of SerRS, equal amounts of GST and each GST-SerRS recombinant protein were separately incubated with recombinant Flag-YY1 overnight and then pulled down by Glutathione resin (Clontech).

Fu C.Y., Wang P.C, & Tsai H.J. (2016). Competitive binding between Seryl-tRNA synthetase/YY1 complex and NFKB1 at the distal segment results in differential regulation of human vegfa promoter activity during angiogenesis. Nucleic Acids Research, 45(5), 2423-2437.

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Quantifying NF-κB p65 DNA Binding

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Nuclear extracts were prepared using the NE-PER Nuclear-Cytoplasmic Extraction Reagents kit (Thermo Fisher Scientific, USA) according to the manufacturer’s protocol. The extracts were stored at − 70 °C, and the protein concentration of the nuclear extracts was quantified by the BCA method (Sigma-Aldrich). NF-κB p65 DNA binding activity was assessed on isolated nuclear extracts by ELISA using the TransAM™ NF-κB Transcription Factor Assay kit according to the manufacturer’s protocol (Active Motif, Carlsbad, CA) [4 (link)]. Each sample was assayed in triplicate. Equal amounts of Raji nuclear extract were used as positive controls.

Chen X., Tang L., Feng J., Wang Y., Han Z, & Meng J. (2017). Downregulation of Paralemmin-3 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Rats by Regulating Inflammatory Response and Inhibiting Formation of TLR4/MyD88 and TLR4/TRIF Complexes. Inflammation, 40(6), 1983-1999.

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