Flies were reared on regular cornmeal diet and housed in standard conditions. The following laboratory fly lines were used: TubP-Gal4, nSyb-Gal4, OK107-Gal4, MB247-Gal4, TubP-Gal80ts; nSyb-Gal4. We obtained the following fly lines from Bloomington Drosophila Stock Center: w1118 (BDSC 3605), NPF-Gal4 (BDSC 25681), TH-Gal4 (BDSC 8848), Gr5a-Gal4 (BDSC 57591), Gr66a-Gal4 (BDSC 57670), UAS-shits (BDSC 44222), Rut2080 (BDSC 9405), Dnc1 (BDSC 6020), UAS-HTT128QFL (BDSC 33808), and UAS-miR-rCD242 (link),43 (link). Gr43aGal4 was obtained from Dr. Hubert Amrein, Texas A&M University9 (link). GMR-Gal4, UAS-41Q-HA, and UAS-41Q-HA were obtained from Dr. Horng-Dar Wang, National Tsing Hua University, Taiwan44 (link). Elav-GeneSwitch (ElavGS) was obtained from Dr. Chun-Hong Chen, National Health Research Institutes. In most cases, flies were reared at 25 °C. For the Gal4/UAS-shits experiments, flies were reared at 23 °C until 1 h before the VBFD assay (32 °C). To express UAS-41Q-HA in adult flies via the TARGET system17 , animals were allowed to develop at 18 °C and transferred to 29 °C right after eclosion.
Uas shits
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2 protocols using uas shits
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Drosophila Behavioral Assays and Genetic Lines
2
Drosophila Genetics and Manipulation
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Flies were reared on regular cornmeal diet and housed in standard conditions. The following laboratory fly lines were used: TubP-Gal4, nSyb-Gal4, OK107-Gal4, MB247-Gal4, TubP-Gal80 ts ; nSyb-Gal4. We obtained the following fly lines from Bloomington Drosophila Stock Center: w 1118 (BDSC 3605), NPF-Gal4 (BDSC 25681), TH-Gal4 (BDSC 8848), Gr5a-Gal4 (BDSC 57591), Gr66a-Gal4 (BDSC 57670), UAS-shi ts (BDSC 44222), Rut 2080 (BDSC 9405), Dnc 1 (BDSC 6020). Gr43a Gal4 was obtained from Dr.
Hubert Amrein, Texas A&M University (9) (link). GMR-Gal4; UAS-41Q-HA and UAS-41Q-HA were obtained from Dr. Horng-Dar Wang, National Tsing Hua University, Taiwan (41) (link). In most cases, flies were reared at 25°C. For the Gal4/UAS-shi ts experiments, flies were reared at 23°C until one hour before the VBFD assay (32°C). To express UAS-41Q-HA in adult flies via the TARGET system ( 16), animals were allowed to develop at 18°C and transferred to 29°C right after eclosion.
Hubert Amrein, Texas A&M University (9) (link). GMR-Gal4; UAS-41Q-HA and UAS-41Q-HA were obtained from Dr. Horng-Dar Wang, National Tsing Hua University, Taiwan (41) (link). In most cases, flies were reared at 25°C. For the Gal4/UAS-shi ts experiments, flies were reared at 23°C until one hour before the VBFD assay (32°C). To express UAS-41Q-HA in adult flies via the TARGET system ( 16), animals were allowed to develop at 18°C and transferred to 29°C right after eclosion.
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