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Anti β actin antibody 2f1 1

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The Anti-β-actin antibody (2F1-1) is a monoclonal antibody that specifically recognizes the β-actin protein, a ubiquitous and highly conserved cytoskeletal protein essential for various cellular processes. This antibody can be used for the detection and quantification of β-actin in different experimental applications.

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Lab products found in correlation

Ab58690, by Abcam (2 mentions) Hrp donkey anti rabbit igg, by BioLegend (2 mentions) Ploy4064, by BioLegend (2 mentions) Proteinase inhibitor cocktail, by Merck Group (2 mentions) Hrp goat anti mouse igg, by BioLegend (2 mentions)

Close spelling variants of this product

Anti-β-actin (27 citations) β-actin (13 citations) Anti-β-actin antibody (6 citations) Anti–β-actin (2F1–1) (4 citations) β-actin antibody (4 citations) Anti-actin (4 citations)

3 protocols using anti β actin antibody 2f1 1

1

Immunoblotting for Cellular Proteins

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Cells were lysed in RIPA buffer with 1% proteinase inhibitor cocktail (Sigma Aldrich) and subjected to SDS-PAGE. Antibodies used for immunoblotting are as follows: anti-β-actin antibody (2F1-1, BioLegend), anti-SLC1A5 antibody (ab58690, abcam), HRP goat anti-mouse IgG, and HRP donkey anti-rabbit IgG (Ploy4064, BioLegend).
Tanaka S., Pfleger C., Lai J.F., Roan F., Sun S.C, & Ziegler S.F. (2018). KAP1 Regulates Regulatory T Cell Function and Proliferation in Both Foxp3-Dependent and -Independent Manners. Cell reports, 23(3), 796-807.
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2

Western Blotting and Tumor Signaling

Cited 1 time
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Western blotting was performed as previously described (Zhang et al., 2014 (link)). Anti-p53 antibody (FL-393 and sc-98, Santa Cruz Biotechnology), anti-SOX2 antibody (D-9, cat#sc-398254, Santa Cruz Biotech), anti-E-FABP antibody (cat#AF1476, R&D systems), and anti-β-actin antibody (2F1-1, cat#643802, Biolegend), were used for overnight incubation (binding). For analysis of p53 expression in primary keratinocytes, skin lysates were immunoprecipitated with anti-p53 antibody (FL-393) before p53 immunoblotting (sc-98). Skin tumors from WT and E-FABP−/−mice (n=3/group) were analyzed using the array for tumor signaling protein and phosphorylation (Full Moon BioSystems, Inc.).
Zhang Y., Hao J., Zeng J., Li Q., Rao E., Sun Y., Liu L., Mandal A., Landers V.D., Morris R.J., Cleary M.P., Suttles J, & Li B. (2018). Epidermal fatty acid binding protein prevents chemical-induced skin tumorigenesis by regulation of TPA-induced IFN/p53/SOX2 pathway in keratinocytes. The Journal of investigative dermatology, 138(9), 1925-1934.
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3

Immunoblotting for Cellular Proteins

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Cells were lysed in RIPA buffer with 1% proteinase inhibitor cocktail (Sigma Aldrich) and subjected to SDS-PAGE. Antibodies used for immunoblotting are as follows: anti-β-actin antibody (2F1-1, BioLegend), anti-SLC1A5 antibody (ab58690, abcam), HRP goat anti-mouse IgG, and HRP donkey anti-rabbit IgG (Ploy4064, BioLegend).
Tanaka S., Pfleger C., Lai J.F., Roan F., Sun S.C, & Ziegler S.F. (2018). KAP1 Regulates Regulatory T Cell Function and Proliferation in Both Foxp3-Dependent and -Independent Manners. Cell reports, 23(3), 796-807.
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