Ifn γ antibody

For neutrophil depletion, mice were treated with 500 μg of anti-Ly6G antibody (clone 1A8, BioLegend, CA) [53 (link)] intraperitoneally 24 and 2 hours prior to infection with lethal inoculum of S. aureus (2 X 10⁸ CFUs/mouse). For IFN-γ inhibition, mice were treated with 100μg of IFN-γ antibody (BioXCell, NH) 12 hours prior to infection with S. aureus. For caspase-1 inhibition, mice were treated with 100 μg of caspase-1 inhibitor (Ac-YVAD-CMK, Cayman chemical, MI) 12 hours prior to infection with S. aureus. For MLKL inhibition, 100 μl of 100 μM MLKL inhibitor (GW806742X, Adipogen, CA) [31 (link)] was injected into each mouse i.p. 12 hours prior to infection with S. aureus. For RIP1 inhibition, mice were treated with 300 μg of necroptosis inhibitor (Nec-1/necrostatin-1s, Calbiochem, MA) [29 (link)] 18 hours before and at the time of bacterial infection, as published elsewhere [29 (link)]. Mice were euthanized to collect BALF and organs 24 hours post infection.