Single-stranded (10-mer) and biotinylated oligonucleotides (10-mer) were purchased from Eurofins Genomics, K.K. (Tokyo, Japan). The chemical reagents, 3,3-dithiodipropionic acid (Nacalai Tesque, Kyoto, Japan), 1-Ethyl-3-[3-(dimethylamino)propyl]carbodiimide (EDC) (Dojindo Laboratories, Kumamoto, Japan), N-Hydroxysuccinimide (NHS) (Wako Pure Chemical Industries, Ltd., Osaka, Japan), and NeutrAvidin (Thermo Fisher Scientific Inc., Tokyo, Japan) were used for experiments with DNA. NaCl (Wako Pure Chemical Industries, Ltd., Osaka, Japan), Tris(hydroxymethyl)aminomethane (Wako Pure Chemical Industries, Ltd., Osaka, Japan), 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES) (Dojindo Laboratories, Kumamoto, Japan), and Ethylenediamine-N,N,N’,N’-tetraacetic acid (EDTA) (Dojindo Laboratories, Kumamoto, Japan) were used to prepare the buffer solutions. Ultrapure water (Milli-Q) was used in all the experiments.
3 3 dithiodipropionic acid
Manufactured by Nacalai Tesque
Sourced in Japan
3,3-dithiodipropionic acid is a chemical compound with the formula (HSCH2CH2)2CO2H. It is a white crystalline solid that is used as a laboratory reagent.
Automatically generated - may contain errors
Lab products found in correlation
2 4 2 hydroxyethyl 1 piperazinyl ethanesulfonic acid hepes, by Dojindo Laboratories (1 mentions)
Ethylenediamine n n n n tetraacetic acid edta, by Dojindo Laboratories (1 mentions)
N hydroxysuccinimide nhs, by Fujifilm (1 mentions)
Tris hydroxymethyl aminomethane, by Fujifilm (1 mentions)
Sodium chloride (nacl), by Fujifilm (1 mentions)
Neutravidin, by Thermo Fisher Scientific (1 mentions)
Tetramethylsilane, by Merck Group (1 mentions)
Succinic anhydride, by Nacalai Tesque (1 mentions)
Amberlite ira 400 chloride form, by Merck Group (1 mentions)
Sodium 3 trimethylsilyl 1 propanesulfonate, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride, by Merck Group (1 mentions)
Sulfuric acid h2so4, by Merck Group (1 mentions)
2 protocols using 3 3 dithiodipropionic acid
1
Functionalization of Oligonucleotides for Biosensors
2
Silane-based Bioconjugation Protocol
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(3-Aminopropyl)triethoxysilane, mercaptopropylisobutylsilsesquioxane (POSS-SH), N-hydroxysuccinimide (NHS), N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC), sodium 3-(trimethylsilyl)-1-propanesulfonate (TMSP), tetramethylsilane (TMS), bovine serum albumin (BSA), 20% hydrogen peroxide (H2O2), 12 M hydrochloride (HCl), Amberlite IRA-400 chloride form, sulfuric acid (H2SO4), deuterium oxide (D2O), deuterated dimethyl sulfoxide-d6 (DMSO-d6), dry methanol (MeOH) were purchased from Sigma-Aldrich (USA). 3,3′-Dithiodipropionic acid and succinic anhydride, were purchased from Nacalai Tesque (Japan). 6-{4-[(1,6-Dioxo-1,6bis(tetradecyloxy)hexan-2-yl)carbamoyl]phenoxy}-N,N,N-trimethylhexan-1-ammonium bromide (calibration lipid) was a gift from Dr. Takayoshi Kawasaki.
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