Hipec

Manufactured by Dow

Sourced in United States

HIPEC is a medical device designed for the delivery of heated, pressurized chemotherapy to the abdominal cavity during surgery. The system heats and circulates the chemotherapy solution within the abdomen, providing direct contact with the target tissues.

Automatically generated - may contain errors

Lab products found in correlation

Axopatch 200 amplifier, by Molecular Devices (2 mentions) Pclamp software, by Molecular Devices (2 mentions)

2 protocols using hipec

Sodium Current Recordings Using Patch Clamp

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Na⁺ currents were recorded using low-resistance, fire-polished patch clamp electrodes (≈ 1–1.5 MΩ) made from 8161 Corning borosilicate glass coated with HIPEC (Dow-Corning, Midland, MI, USA) to minimize electrode capacitance. An Axopatch 200 amplifier and pClamp software (Molecular Devices, Sunnyvale, CA, USA) were used to record Na⁺ currents. The series resistance was compensated to 80% to minimize voltage-clamp errors. The cells were allowed to stabilize for 5 min after the whole-cell configuration was established. The membrane potential was held at − 140 mV before the currents were recorded. Sodium currents were filtered at 5 kHz and were digitized at 83.33 kHz. The liquid junction potential was not corrected. All the experiments were performed at room temperature (22 °C).

Plumereau Q., Ebdalla A., Poulin H., Appendino J.P., Scantlebury M.H., Au P.Y, & Chahine M. (2022). De novo Y1460C missense variant in NaV1.1 impedes the pore region and results in epileptic encephalopathy. Scientific Reports, 12, 17182.

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Patch-Clamp Recordings of Sodium Currents

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Na⁺ currents were recorded using low-resistance, fire-polished electrodes (≈1 MΩ) made from 8161 Corning borosilicate glass coated with HIPEC (Dow-Corning, Midland, MI) to minimize electrode capacitance. An Axopatch 200 amplifier and pClamp software (Molecular Devices, Sunnyvale, CA) were used to record Na⁺ currents. The series resistance was compensated to 80% to minimize voltage-clamp errors. The cells were allowed to stabilize for 5 minutes after the whole-cell configuration was established. The membrane potential was held at –140 mV before the currents were recorded. Sodium currents were filtered at 5 kHz and digitized at 83.33 kHz. The liquid junction potential was not corrected. All the experiments were performed at room temperature (22 °C).

Plumereau Q., Theriault O., Pouliot V., Moreau A., Morel E., Fressart V., Denjoy I., Delinière A., Bessière F., Chevalier P., Gamal El-Din T.M, & Chahine M. (2020). Novel G1481V and Q1491H SCN5A Mutations Linked to Long QT Syndrome Destabilize the Nav1.5 Inactivation State. CJC Open, 3(3), 256-266.

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