Total RNA samples were analyzed by CapitalBiotech (CapitalBio Corp.) for microarray experiments. The Agilent human miRNA Array V21.0 (8 × 60 K) was applied for detection of profiling of miRNA in the serum specimens of 12 NPC patients with different radiosensitivity. Totally, the microarray covered 2,549 miRNAs from the latest miRBase database. The slide was scanned by the Agilent Microarray Scanner (Cat# G2565CA, Agilent technologies, Santa Clara, CA, US) and the Feature Exaction software 10.7 (Agilent technologies, Santa Clara, CA, US)16 (link). The miRNA array data were analyzed for data summarization, normalization and quality control by using the GeneSpring software V13 (Agilent). The default 90th percentile normalization method were performed for date preprocessing. All steps were performed were according to the recommended protocol. To screen out the candidate miRNAs, threshold values of ≥2 and ≤−2 fold change and a Benjamini-Hochberg corrected p vlaue of 0.05 were used. The data was Log2 transformed and median centered by genes using the Adjust Data function of CLUSTER 3.0 software. Then we further analyzed the data with hierarchical clustering with average linkage. Finally, we performed tree visualization by using Multiple Experiment Viewer (MeV version 4_9_0).
Feature exaction software 10
Manufactured by Agilent Technologies
Sourced in United States
Feature Exaction software 10.7 is a data analysis tool designed for use with Agilent Technologies' lab equipment. The software's core function is to extract and analyze specific features from data collected during laboratory experiments or analyses.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using feature exaction software 10
1
Profiling of miRNA in NPC Radiosensitivity
2
Profiling miRNA in Hepatocellular Carcinoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Agilent human miRNA microarray release 21.0 (8 × 60 K) was applied for detection of profiling of miRNA in 7 pairs of HCC and matched tumor-free tissues. Totally, the of microarray covered 2,549 miRNAs from the latest miRBase database. The slide was scanned by the Agilent Microarray Scanner (Cat# G2565CA, Agilent technologies, Santa Clara, CA, US) and the Feature Exaction software 10.7 (Agilent technologies, Santa Clara, CA, US). Raw data from Scanner were normalized by Quantile algorithm, Gene Spring Software. All of the performances were according to the manufacturer's protocol. To screen out the significantly differentially expressed miRNAs, the threshold of P < 0.01 and fold change ≥ 2 or ≤ 0.5 was set up. The data have been deposited in NCBI's Gene Expression Omnibus and are accessible through GEO Series accession number GSE108724 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc =GSE108724).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!