106 cells in 100 μL FACS buffer were stained with fluorochrome-labelled antibodies for macrophage type-specific cell surface markers: anti-mouse CD23 (cat. # 101618, clone B3B4, Per.CP/Cy5.5, 7 μl, Biolegend, CA), anti-mouse CD38 (cat. # 102705, clone 90, FITC, 3 μl, Biolegend, San Diego, CA), anti-mouse CD80 (cat. # 104707, clone 16–10A1, PE, 3 μl, Biolegend, CA), anti-mouse CD206 (cat. # 141708, clone C068C2, APC, 3 μl, Biolegend, CA), anti-human CD80 (cat. # 305208, clone 2D10, PE, 10 μl, BD Pharmingen, CA), anti-human CD86 (cat. # 555660, clone 2331, APC, 10 μl, BD Pharmingen, CA), anti-human CD163 (cat. # 326512, clone RM3/1, PerCP-Cy5.5, 15 μl, BD Pharmingen, CA), anti-human CD206 (cat. # 551135, clone 19.2, FITC, 10 μl, BD Pharmingen, CA). Cell counts were obtained before flow staining by trypan blue exclusion and normalized to single stained and isotype controls on a FACSCanto II (BD Biosciences) or BD Accuri c6 (BD Biosciences) and FlowJo Ver. 10 software (Tree Star, Ashland, OR, USA) or BD FACS Xpress software were used for analyses.
Anti human cd206
Manufactured by BD
Sourced in United States
Anti-human CD206 is a laboratory reagent used for the detection and analysis of the CD206 receptor, also known as the macrophage mannose receptor, on the surface of human cells. CD206 is a type I transmembrane protein that plays a role in the endocytosis of glycoproteins. This reagent can be used in various immunological and cell biology applications, such as flow cytometry, to identify and characterize cells expressing the CD206 receptor.
Automatically generated - may contain errors
Lab products found in correlation
Percp cy5, by BioLegend (1 mentions)
Anti mouse cd38, by BioLegend (1 mentions)
Anti human cd80, by BioLegend (1 mentions)
Anti human cd86, by BD (1 mentions)
Anti mouse cd206, by BioLegend (1 mentions)
Anti human cd163, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
Accuri c6, by BD (1 mentions)
Facsaria 3, by BD (1 mentions)
Facscalibur, by Beckman Coulter (1 mentions)
3 protocols using anti human cd206
1
Macrophage Phenotyping by Flow Cytometry
2
Flow Cytometric Macrophage Phenotyping
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Macrophages were resuspended in staining medium (phenol-red free DMEM, 10 mM HEPES, 2 % fetal bovine serum) and incubated with anti-mouse CD206 (AbD Serotec), anti-human CD206 (BD Biosciences), or corresponding unspecific isotype control antibodies for 20 minutes at 4 °C. Cells were washed and resuspended in staining medium for flow cytometry. Data acquisition was performed using a FACSAria III instrument (BD Biosciences) and data were analyzed with FlowJo software.
3
Macrophage Surface Marker Analysis
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The macrophages were washed with cold PBS and separated with 5 mM ethylene diamine tetraacetic acid. Then, the macrophages (0.5 × 106) were resuspended in 1 mL PBS, added with fluorescein isothiocyanate, phycoerythrin and allophycocyanin conjugated Abs anti-human CD206 (BD Pharmingen, USA). FACScalibur (Beckman Coulter, USA) was used for flow cytometry analysis.
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