Mouse monoclonal anti α tubulin clone dm 1a

Following antibodies and dilutions were used: polyclonal rabbit anti-ARHGEF6/αPIX (Cat. No. HPA003578; WB 1:400) from Atlas Antibodies, Stockholm, Sweden; and anti-EEA1 (early endosome antigen 1; Cat. No. 610456; IF 1:500) from BD Biosciences, Heidelberg, Germany; rabbit polyclonal anti-Cbl (C-15; Cat. No. sc-170; WB 1:1000; IF 1:200), rabbit polyclonal anti-EGFR (clone 1005; Cat. No. sc-03; WB 1:300, IF 1:200) and mouse monoclonal anti-EGFR (clone R-1; Cat. No. sc-101; IF 1:200) from Santa Cruz, Heidelberg, Germany; mouse monoclonal anti-GAPDH (Cat. No. ab8245; WB 1:10000) from Abcam, Cambridge, UK; mouse monoclonal anti-α-Tubulin (clone DM 1A; Cat. No. T9026; WB 1:7500); rabbit polyclonal anti-HA (Cat. No. H6908; IF 1:200) and mouse monoclonal anti-FLAG M2 Peroxidase Conjugate (Cat. No. A8592; WB 1:3000) from Sigma-Aldrich, Taufkirchen, Germany; rat monoclonal anti-HA-HRP (Cat. No. 2013819; WB 1:4000) from Roche, Mannheim, Germany; mouse monoclonal anti-V5-HRP (Cat. No. R96125; WB 1:5000) from Life Technologies, Darmstadt, Germany; epidermal growth factor (EGF) complexed with Alexa Fluor 488 was purchased from Molecular Probes/Life Technologies, Darmstadt, Germany and unlabelled human epidermal growth factor from Sigma-Aldrich, Taufkirchen, Germany.