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Pick mix pcr pre designed panels

Manufactured by Qiagen
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Sourced in Switzerland

Pick&Mix PCR pre-designed panels are a collection of optimized primer and probe sets for targeted gene expression analysis. They provide a flexible and customizable solution for quantitative PCR (qPCR) experiments.

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Lab products found in correlation

Lightcycler 480, by Roche (3 mentions) Exilent sybr green master mix, by Qiagen (3 mentions) Mircury lnatm universal cdna synthesis kit 2, by Qiagen (2 mentions) Mircury lna universal cdna synthesis kit 2, by Qiagen (1 mentions)

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Pick-&-Mix panel (2 citations)

3 protocols using pick mix pcr pre designed panels

1

miRNA Expression Profiling: A Three-Year Validation

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MiRNA was reverse-transcribed using the MiRCURY LNATM Universal cDNA Synthesis Kit II (Exiqon, Vedbaek, Denmark) according to the manufacturer’s protocol. After adjusting RNA concentration to 5 ng/µL and mixing with reaction buffer and enzyme mix, a retro-transcription reaction was carried out at 42 °C for 60 min. Artificial RNA UniSp6 from the same kit was used as a retro-transcription control. Quantitative PCR (qPCR) was performed on a LightCycler 480 (Roche, Basel, Switzerland) using miRNA LNA technology and Pick&Mix PCR pre-designed panels (Exiqon, Vedbaek, Denmark) with miRNA UniSp3 as interplate calibrator control. cDNA was diluted 1:80, 4 µL were used with ExiLENT SYBR Green Master Mix (Exiqon, Vedbaek, Denmark) following manufacturer’s indications and samples were set up in duplicate.
The validation study was carried out in three independent phases, the first in 2017, the second in 2018 and the third in 2019, including the subjects as described in Section 2.1.
Gámez-Valero A., Campdelacreu J., Vilas D., Ispierto L., Gascón-Bayarri J., Reñé R., Álvarez R., Armengol M.P., Borràs F.E, & Beyer K. (2021). Platelet miRNA Biosignature Discriminates between Dementia with Lewy Bodies and Alzheimer’s Disease. Biomedicines, 9(9), 1272.
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2

miRNA Reverse Transcription and qPCR Validation

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MiRNA was reverse-transcribed using the MiRCURY LNA TM Universal cDNA Synthesis Kit II (Exiqon, Vedbaek, Denmark) according to the manufacturer's protocol.
After adjusting RNA concentration to 5 ng/µL and mixing with reaction buffer and enzyme mix, a retro-transcription reaction was carried out at 42ºC for 60 minutes.
Artificial RNA UniSp6 from the same kit was used as a retro-transcription control.
Quantitative PCR (qPCR) was performed on a LightCycler 480 (Roche, Basel, Switzerland) using miRNA LNA technology and Pick&Mix PCR pre-designed panels (Exiqon) with miRNA UniSp3 as interplate calibrator control. cDNA was diluted 1:80, 4 µL were used with ExiLENT SYBR Green Master Mix (Exiqon, Vedbaek, Denmark) following manufacturer's indications and samples were set up in duplicate.
The validation study was carried out in three independent phases, the first in 2017, the second in 2018 and the third in 2019, including the subjects as described in 2.1.
Gámez‐Valero A., Campdelacreu J., Vilas D., Ispierto L., Samaniego D., Gascón‐Bayarri J., Reñé R., Alvárez R., Armengol M.P., Borràs F.E., & Beyer K. (2020). Platelet miRNA bio-signature discriminates between dementia with Lewy bodies and Alzheimer disease.
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3

MiRNA Quantification by qPCR: A Comprehensive Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols
MiRNA was reverse-transcribed using the MiRCURY LNA ™ Universal cDNA Synthesis Kit II (Exiqon, Vedbaek, Denmark) according to the manufacturer's protocol. After adjusting RNA concentration to 5 ng/µL and mixing with reaction buffer and enzyme mix, a retro-transcription reaction was carried out at 42ºC for 60 minutes. Artificial RNA UniSp6 from the same kit was used as a retro-transcription control. Quantitative PCR (qPCR) was performed on a LightCycler 480 (Roche, Basel, Switzerland) using miRNA LNA technology and Pick&Mix PCR pre-designed panels (Exiqon) with miRNA UniSp3 as interplate calibrator control. cDNA was diluted 1:80, 4 µL were used with ExiLENT SYBR Green Master Mix (Exiqon, Vedbaek, Denmark) following manufacturer's indications and samples were set up in duplicate.
The validation study was carried out in three independent phases, the first in 2017, the second in 2018 and the third in 2019, including the subjects as described in 2.1.
Gámez‐Valero A., Campdelacreu J., Vilas D., Ispierto L., Samaniego D., Gascón‐Bayarri J., Reñé R., Alvárez R., Armengol M.P., Borràs F.E., & Beyer K. (2020). Platelet miRNA bio-signature discriminates between dementia with Lewy bodies and Alzheimer disease: A cross-sectional study.
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