Total RNA extracted from the SW480 cells using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. The quality of the RNAs was assessed using Nanodrop 2000 spectrophotometry (Thermo Fisher Scienti c, Inc), after which the samples were sent to Vazyme Biotech (Nanjing, China) for RNA-sequencing.
Bioinformatics, plasmid construction, and dual-luciferase reporter assay.
The target of miRNA-3934 was obtained from starBase (Version 3.0) prediction. GenePharma synthesized a plasmid containing the sequences of miRNA-3934 paired with the RAGE 3'-UTR (RAGE-wild type (WT)) or mutant 3'-UTR (RAGE-mutant type (MUT)) regions. The miRNA-3934 mimics and RAGE (GenePharma) were co-transfected into 293T cells. Luciferase activity was then examined 48 h after transfection using the dual-luciferase reporter assay kit (Beyotime, Shanghai, China).
Bioinformatics, plasmid construction, and dual-luciferase reporter assay.
The target of miRNA-3934 was obtained from starBase (Version 3.0) prediction. GenePharma synthesized a plasmid containing the sequences of miRNA-3934 paired with the RAGE 3'-UTR (RAGE-wild type (WT)) or mutant 3'-UTR (RAGE-mutant type (MUT)) regions. The miRNA-3934 mimics and RAGE (GenePharma) were co-transfected into 293T cells. Luciferase activity was then examined 48 h after transfection using the dual-luciferase reporter assay kit (Beyotime, Shanghai, China).