Ftir spectroscope

Fourier Transform Infrared Spectrophotometer (FTIR) is a tool for identifying the types of functional groups present in compounds. The wavelength of light absorbed is characteristic of the chemical bond seen in the annotated spectrum. By interpreting the infrared absorption spectrum, the chemical bonds in a molecule can be determined. Dried powder of different extract of purified fraction of Cucurbita pepo seed was used for FTIR analysis. 10 mg of the dried extract powder was encapsulated in 100 mg of potassium bromide (KBr) pellet, to prepare sample discs. The thin pressed pellet was loaded in FTIR spectroscope (Agilent) and infrared spectra and peak values were recorded between 4,000-600 cm -1 . For each sample, 200 scans were averaged with a spectral resolution of 4 cm -1 . It took three minutes for a recording process. Then for a given material, a final average spectrum was calculated (Kumar and Ramaswamy, 2014) .

Pure sample of each of the agricultural waste adsorbent in powder form was analysed using Agilent FTIR spectroscope (range: 4000-650). To do this 1 g of each pure sample was measured using the weighing balance and analyze with the FTIR spectroscope using ASTM E1252-98 standard method. The FTIR analysis was carried out at the central laboratory of Yaba College of Technology, Lagos, Nigeria. In the FTIR analysis, an Infra Red (IR) light source passed through the sample onto a detector, which precisely measures the amount of light absorbed by the sample. The absorbance created a unique spectral fingerprint that was used to identify the molecular structure of the sample and determine the exact quality of a particular compound in the sample.