Pupal and adult eye–brain complexes were dissected in cold Schneider’s Drosophila medium and fixed in 4% paraformaldehyde in phosphate-buffered saline for 40 min. Tissues were washed in Phosphate-buffered saline + 0.4% Triton-X (PBST) and mounted in Vectashield (Vector Laboratories, CA). Images were obtained with a Leica TCS SP8-X white laser confocal microscope with a ×63 glycerol objective (c = 1.3). The primary antibodies used in this study with given dilutions were as follows: mouse monoclonal anti-Chaoptin (1:200; Developmental Studies Hybridoma Bank); rat monoclonal anti-nCadherin (1:100; Developmental Studies Hybridoma Bank); rabbit monoclonal anti-Atg8 (1:100; Abcam); mouse monoclonal anti-Trio (1:50; Developmental Studies Hybridoma Bank); mouse monoclonal anti-LAR (1:50; Developmental Studies Hybridoma Bank); goat polyclonal anti-GFP (1:1000; Abcam); rat monoclonal anti-GFP (1:500; BioLegend); rabbit polyclonal anti-CD4 (1:600; Atlas Antibodies); rabbit polyclonal anti-DsRed (1:500; ClonTech); rabbit anti-Syd-1 (1:500; gift from Sigrist Lab). The secondary antibodies Cy3, Cy5 (Jackson ImmunoResearch Laboratories) and Alexa488 (Invitrogen) were used in 1:500 dilution.
Rabbit polyclonal anti cd4
Manufactured by Atlas Antibodies
Rabbit polyclonal anti-CD4 is a primary antibody produced in rabbits that binds to the CD4 protein. CD4 is a cell surface glycoprotein expressed on a subset of T cells and plays a role in the immune response.
Automatically generated - may contain errors
Lab products found in correlation
Rat monoclonal anti ncadherin, by Developmental Studies Hybridoma Bank (2 mentions)
Goat polyclonal anti gfp, by Abcam (2 mentions)
Rat monoclonal anti gfp, by BioLegend (2 mentions)
Rabbit polyclonal anti dsred, by Takara Bio (2 mentions)
Vectashield, by Vector Laboratories (2 mentions)
Cyanine3 (cy3), by Jackson ImmunoResearch (2 mentions)
Tcs sp8 x white laser confocal microscope, by Leica (1 mentions)
Alexa 488, by Thermo Fisher Scientific (1 mentions)
Alexa 488, by Jackson ImmunoResearch (1 mentions)
2 protocols using rabbit polyclonal anti cd4
1
Immunofluorescent Staining of Drosophila Eyes
2
Immunohistochemical Analysis of Drosophila Eye-Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immunohistochemistry and fixed imaging Pupal and adult eye-brain complexes were dissected in cold Schneider's Drosophila medium and fixed in 4% paraformaldehyde (PFA) in PBS for 40 minutes. Tissues were washed in PBST (0.4% Triton-X) and mounted in Vectashield (Vector Laboratories, CA). Images were obtained with a Leica TCS SP8-X white laser confocal microscope with a 63X glycerol objective (NA = 1.3). The primary antibodies used in this study with given dilutions were as follows: rat monoclonal anti-nCadherin (1:100; Developmental Studies Hybridoma Bank); goat polyclonal anti-GFP (1:1000; Abcam); rat monoclonal anti-GFP (1:500; BioLegend); rabbit polyclonal anti-CD4 (1:600; Atlas Antibodies); rabbit polyclonal anti-DsRed (1:500; ClonTech). The secondary antibodies Alexa488, Cy3, Cy5 (Jackson ImmunoResearch Laboratories) were used in 1:500 dilution.
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