Bromelain

Bovine pancreatic trypsin, bovine pancreatic chymotrypsin, papain from papaya tree latex, and bromelain from pineapple stem were procured from Sisco Research Laboratory (Mumbai, India). Bovine serum albumin (BSA), ficin (fig tree latex), N-α-benzoyl-dl-arginine-p-nitroanilide (BApNA), N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide (SAApNA), pGlu-Phe-Leu-p-nitroanilide (pFLNA), N-acetyl-d-glucosamine, laminarin, crab shell chitin, and dithiotheitol (DTT) were purchased from Sigma (St. Louis, MO). Glucose estimation kit procured from Kamineni Life Sciences, Hyderabad. X-ray films were purchased from Carestream Health India Pvt Ltd. (Mumbai). All other chemicals and reagents used were of analytical grade. 1). All these lines were biofortified using classical recombinant breeding approaches (Govindaraj et al. 2019a) . Briefly, biofortification was achieved by crossing among the high Fe/Zn lines and later to the elite lines followed by pedigree breeding to fix the line with high Fe/Zn in agronomically superior backgrounds at F 6 and later stages. These micronutrients are highly heritable and predominantly controlled by additive genes, and therefore, it is highly feasible to breed biofortified lines in pearl millet. Pearl millet biofortification breeding approaches are well described elsewhere (Rai et al. 2012; Govindaraj et al. 2013 Govindaraj et al. , 2019b)) .

The wine samples were purchased from the local market. The brand was -Samara White Wine (Alcohol content: 11% v/v) manufactured by Sula Vineyards Pvt. Ltd., Nashik, Maharashtra, India. The natural components-Bromelain, Quercetin, Ascorbic acid and Histamine (standard) were purchased from Sisco Research Laboratories Pvt Ltd with other consumables.