Diethylene glycol deg

Dextran (MW ~70,000), chitosan (medium molecular weight), l-cysteine,
N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide
hydrochloride (EDAC), dialysis tubing (molecular weight cut-off (MWCO) 12,000
Da), phalloidin–fluorescein isothiocyanate (FITC), 4′,6-diamidino-2-phenylindole
(DAPI) stain, PVA-DABCO^® antifadding agent, 2′,7′-dichlorofluorescein
diacetate (DCFDA), and doxorubicin hydrochloride were purchased from
Sigma-Aldrich, USA. Anti-collagen mouse mAb and cy5-tagged anti-mouse secondary
antibody were purchased from Cell Signalling Technology, Inc. Sodium periodate,
diethylene glycol (DEG), and 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) were
procured from SRL chemicals, India. Lung adenocarcinoma A549 cells were obtained
with passage no 21 from NCCS, India. Fetal bovine serum (FBS) was procured from
Gibco, USA. Bovine serum albumin (BSA), Roswell Park Memorial Institute
(RPMI)-1640, and penicillin–streptomycin antibiotic solution were purchased from
Himedia, India. LIVE/DEAD^® Viability/Cytotoxicity Kit was purchased
from Thermo Fisher Scientific, India.

Locust bean gum (from Ceratonia siliqua seeds, Molecular Weight ∼ 310 kDa, M/G = 4:1), chitosan (Molecular weight 190–310 kDa and Degree of deacetylation 75–85%), dextran (from Leuconostoc ssp., Mr (Relative molecular mass) ∼ 70 kDa), L-cysteine, N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDAC), dialysis tubing cellulose membrane (MWCO ∼ 12,000 Da), were purchased from Sigma –Aldrich. 5, 5′-dithio-bis-(2-nitrobenzoic acid) (DTNB), diethylene glycol (DEG) and sodium periodate were purchased from SRL chemicals, India. Mouse embryonic fibroblast 3T3-L1 cells were received from NCCS Pune, India. DMEM and Fetal bovine serum (FBS) were purchased from Gibco, USA.