Apoptotic cells were detected by using the Annexin-V-APC/PI double staining kit (KeyGen Biotech, China). After detaching the verteporfin-treated cells from the six-well plate with 0.25% trypsin (without EDTA) (Gibco, USA), they were washed twice with PBS and centrifuged (2000 g, 5 min). Cells were then resuspended in binding buffer and stained with Annexin-V-APC and PI for 30 min in the dark. Finally, we detected stained cells by using flow cytometry and analyzed apoptosis with FlowJo X software.
Annexin 5 apc pi double staining kit
Manufactured by Keygen Biotech
Sourced in China
The Annexin-V-APC/PI double staining kit is a laboratory tool used to detect and quantify apoptotic cells. It combines two fluorescent dyes, Annexin-V-APC and Propidium Iodide (PI), to distinguish between early apoptotic, late apoptotic, and necrotic cells during flow cytometry analysis.
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2 protocols using annexin 5 apc pi double staining kit
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Annexin-V-APC/PI Apoptosis Assay
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Apoptosis Detection by Annexin-V-APC/PI
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An Annexin-V-APC/PI double-staining kit (KeyGen Biotech Co., Ltd., China) was used to detect apoptotic cells according to the manufacturer’s protocol. After treated cells were detached from culture plates with 0.25% trypsin (without EDTA) (Gibco, Carlsbad, CA, USA), we washed them twice with phosphate-buffered saline (PBS) and centrifugation (2000 x g, 25 °C, 5 min). Then, the cells were resuspended in binding buffer and stained with both annexin V-APC and PI for 15 min in the dark. Finally, we used flow cytometry (BD FACSCalibur™ cytometer, BD Biosciences) to detect stained cells.
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