C57BL/6NHsd mice, 11-12 weeks of age (Envigo), were anesthetized with 4% isoflurane and secured in a stereotaxic head frame (Harvard Apparatus, Holliston, MA). GL-261-Red-Fluc cells (1×105 cells suspended in 2 μl of PBS) were injected into the brain parenchyma (coordinates: 3 mm anterior to bregma; 1.5 mm lateral to sagittal suture; 3 mm down from surface) through a burr hole in the skull using a 10-μl Hamilton syringe. Biophotonic images of the skull were captured using a Xenogen IVIS 200 imaging system (Palo Alto, CA) 2 weeks after initial cell implantation (Figure 6 C). Prior to imaging, each mouse received an intraperitoneal injection of 100 μl of D- luciferin (30 mg/ml solution; PerkinElmer, Waltham, MA) and was anesthetized by isoflurane inhalation. The resulting images were evaluated, and luminescence measurements from equivalent regions of interest encompassing the entire skull were collected using Living Image 4.1 software (Xenogen).
Stereotaxic head frame
Manufactured by Harvard Apparatus
The Stereotaxic Head Frame is a precise and adjustable instrument used for securing the head of an animal during neuroscience research procedures. It provides a stable platform to position the animal's head in specific coordinates, enabling accurate targeting and manipulation of specific brain regions.
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Lab products found in correlation
2 protocols using stereotaxic head frame
1
Bioluminescent Glioma Tumor Imaging
2
Intracranial Glioblastoma Implantation in Mice
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Intracranial Injection of Mice with U87MG-Luciferase Glioblastoma Cells: Intracranial implantation of mice was performed as previously described (Wilk et al., (2014) Mol. Cell Biol. 35: 182-198; Marrero et al., (2014) Neoplasia 16: 874-882, incorporated herein by reference in their entireties). Briefly, female nude-Foxnlnu athymic mice, 6-8 weeks of age (Harlan Laboratories) were anesthetized with 4% isoflurane and secured in a stereotaxic head frame (Harvard Apparatus, Holliston, Mass.). The tumor cells (3×104 in 2 μL of artificial cerebrospinal fluid) were injected into the left striatum through a burr hole in the skull using a 10 μl Hamilton syringe.
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