Hec1A, AGS, and HCC1500 cells were acquired from ATCC. All other cell lines (HCC1806, SUM149, BT549, HCC1937, HCC70, BT20, MCF7, MDAMB453, EFM19, ZR75-1, and T47D) were acquired from the Brugge Lab at Harvard Medical School, and were authenticated by STR analysis. All cell lines were of female origin. Cell lines were regularly tested for mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza). Cells were grown in human plasma-like medium according to the published formulation (Cantor et al., 2017 (link)) with 5% dialyzed FBS (Sigma) and Pen/Strep (Invitrogen) at 37 degrees C with 5% CO2. Media was changed at least every two days. As needed, cells were incubated in RPMI media (R9010-01, US Biological Life Sciences) with or without serine and glycine with 5% dialyzed FBS and Pen/Strep. Cells were counted using a Z1 Coulter Particle Counter (Beckman Coulter) and growth rates were calculated using the following formula: growth rate = ln(final cell number/initial cell number)/time.
Pen strep
Manufactured by US Biological
Pen/Strep is a sterile solution containing the antibiotics penicillin and streptomycin. It is commonly used in cell culture applications to prevent bacterial contamination.
Automatically generated - may contain errors
Lab products found in correlation
Pen strep, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Fetal bovine serum (fbs), by US Biological (2 mentions)
Hcc1500, by American Type Culture Collection (2 mentions)
Z1 coulter particle counter, by Beckman Coulter (2 mentions)
Mycoalert mycoplasma detection kit, by Lonza (2 mentions)
Rpmi media, by US Biological (2 mentions)
2 protocols using pen strep
1
Culture of Breast Cancer Cell Lines
2
Culture of Breast Cancer Cell Lines
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Hec1A, AGS, and HCC1500 cells were acquired from ATCC. All other cell lines (HCC1806, SUM149, BT549, HCC1937, HCC70, BT20, MCF7, MDAMB453, EFM19, ZR75-1, and T47D) were acquired from the Brugge Lab at Harvard Medical School, and were authenticated by STR analysis. All cell lines were of female origin. Cell lines were regularly tested for mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza). Cells were grown in human plasma-like medium according to the published formulation (Cantor et al., 2017 (link)) with 5% dialyzed FBS (Sigma) and Pen/Strep (Invitrogen) at 37 degrees C with 5% CO2. Media was changed at least every two days. As needed, cells were incubated in RPMI media (R9010-01, US Biological Life Sciences) with or without serine and glycine with 5% dialyzed FBS and Pen/Strep. Cells were counted using a Z1 Coulter Particle Counter (Beckman Coulter) and growth rates were calculated using the following formula: growth rate = ln(final cell number/initial cell number)/time.
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