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Tocopherol homologues

Manufactured by Merck Group
Sourced in Germany

Tocopherol homologues are a group of naturally occurring compounds that are chemically similar to vitamin E. They are found in various plant-based oils and are commonly used in laboratory settings as reference standards or research materials.

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3 protocols using tocopherol homologues

1

Antioxidant Compound Characterization

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2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2-diphenyl-1-picrylhydrazyl (DPPH), isoflavone and phytosterol standards were from Sigma (Sigma Chemical Co., St. Louis, MO, USA). Tocopherol homologues were from Calbiochem-Merck Biosciences (Darmstadt, Germany). Methanol and acetonitrile (HPLC purity) were from POCh (Gliwice, Poland). Water was purified with the Milli-Q-system (Millipore, Bedford, USA).
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2

Quantification of Phenolic Acids and Tocopherols

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Phenolic acid standards were purchased from Sigma (St. Louis, MO). All tocopherol homologues (purity > 95% by HPLC) were obtained from Calbiochem-Merck Biosciences (Darmstadt, Germany). n-Hexane (HPLC-grade) and 1.4-dioxane (HPLC-grade) were purchased from Merck (Darmstadt, Germany). A fluorescent probe NBD-PE (N-(7-nitrobenz-2-oxa--1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3--phosphoethanolamine, triethylammonium salt) was acquired from Thermo Fisher Scientific (USA). All other solvents and chemicals used in this study were of analytical grade.
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3

Extraction and Characterization of Tocopherol Homologues

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All tocopherol homologues (purity > 95% by HPLC) were obtained from Calbiochem-Merck Biosciences (Darmstadt, Germany). n-Hexane (HPLC-grade), and 1.4-dioxane (HPLC-grade) were purchased from Merck (Darmstadt, Germany). All other solvents and chemicals used in this study were of analytical grade.
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