The ovaries were quickly collected, cleaned of fat, fixed in 4% paraformaldehyde, dehydrated in 70% ethanol and embedded in paraffin. Then, the ovaries were serially sectioned into 5 μm thick sections (LEICA CM1850, Germany) and stained with hematoxylin and eosin (Beisuo Biotech Company, China). Follicles were counted in every fifth section and categorized into different developmental stages based on standards of classification.35 (link) Immunohistochemistry was performed as described previously.36 (link) The primary antibodies included anti-CYP19A1 (ab18995; Abcam) and anti-3β-HSD (sc-515120; Santa Cruz).
Sc 515120
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Sc-515120 is a lab equipment product from Santa Cruz Biotechnology. It is a device designed for scientific research applications. The core function of this product is to facilitate various laboratory procedures, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Cm1850, by Leica (1 mentions)
Ab18995, by Abcam (1 mentions)
Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (1 mentions)
Df6894, by Affinity Biosciences (1 mentions)
T0004, by Affinity Biosciences (1 mentions)
Ab125022, by Abcam (1 mentions)
Ab1431, by Abcam (1 mentions)
Ab128908, by Abcam (1 mentions)
Ab9324, by Abcam (1 mentions)
Gtx56293, by GeneTex (1 mentions)
Ab9722, by Abcam (1 mentions)
Ab188224, by Abcam (1 mentions)
2 protocols using sc 515120
1
Ovarian Follicle Developmental Analysis
2
Protein Extraction and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total protein was extracted from tissues or cells using RIPA lysis buffer (CW2333, CWBIO, China) containing proteinase and phosphatase inhibitors. Nuclear and cytoplasmic protein of TM3 cells was extracted using NE-PER Nuclear and Cytoplasmic Extraction Reagents (78833, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions. Western blot analysis was conducted as previously described [35 (link)]. The primary antibodies included anti-FOXO4 (ab128908, 1:1000; Abcam, USA), anti-p53 (ab26, 1:200; Abcam, USA), anti- Ser15-phospho-p53 (ab1431, 1:500; Abcam, USA), anti-p21 (ab188224, 1:1000; Abcam, USA), anti-p16 (MAB2416, 1:500; Abnova, USA), anti-3β-HSD (sc-515120, 1:200; Santa Cruz, USA), anti-CYP11A1 (GTX56293, 1:500; Gene Tex, USA), anti-CYP17A1 (ab125022, 1:1000; Abcam, USA), anti-IL-1α (16764-1-AP, 1:500; Proteintech, USA), anti-IL-1β (ab9722, 0.2 μg/ml; Abcam, USA), anti-IL-6 (ab9324, 0.4 μg/ml; Abcam, USA), anti-IL-10 (DF6894, 1:500; Affinity, USA), anti-TNF-α (17590-1-AP, 1:500; Proteintech, USA), anti-TGF-β (18978-1-AP, 1:500; Proteintech, USA) and anti-GAPDH (T0004, 1:5000; Affinity, USA) antibodies. GAPDH was used as the control.
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