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NUAK1 is a protein kinase that is part of the AMPK-related kinase family. It plays a role in regulating cellular processes such as cell growth, proliferation, and metabolism.

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Lab products found in correlation

Mab3211, by Merck Group (2 mentions) Bay 11 7082, by Cayman Chemical (2 mentions) Nf κb2, by Cell Signaling Technology (2 mentions) C rel, by Cell Signaling Technology (2 mentions) Antibody against tubulin t5168, by Merck Group (2 mentions) Rela s536 p, by Cell Signaling Technology (2 mentions) A2066, by Merck Group (2 mentions) Hrp conjugated antibodies against mouse igg na931, by Cytiva (2 mentions) Rabbit igg na934, by Cytiva (2 mentions) Ros glo h2o2 assay, by Promega (2 mentions) Wnt5a b, by Cell Signaling Technology (1 mentions) P mek1 2, by Cell Signaling Technology (1 mentions) P s6k, by Cell Signaling Technology (1 mentions) Paccs79, by Cell Signaling Technology (1 mentions) Flag polyclonal, by Merck Group (1 mentions) Total erk1 2, by Cell Signaling Technology (1 mentions) Total acc, by Cell Signaling Technology (1 mentions) P p90rsks380, by Cell Signaling Technology (1 mentions) P ulk1s555, by Cell Signaling Technology (1 mentions) P paxillin y118, by Cell Signaling Technology (1 mentions)

3 protocols using nuak1

1

Signaling Pathway Antibody Analysis

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Cell Signaling Technology antibodies used: Wnt5a/b (#2530), Snail (#3879), Slug (#9585), ZEB1 (#3396), LKB1 (#3047), P-ACC S79 (#3661), Total ACC (#4190), Axin2 (#2151), MARK2 (#9118), MARK3 (#9311), AMPKalpha (#2532), P-ULK1 S555 (#5869), Nuak1 (#4458), SIK2 (#6919), GST (#2622), myc-tag (#2272), P-Src family Y416 (#2113), Src (#2109), P-Paxillin Y118 (#2541), Pathscan I for P-ERK1/2 and P-Akt S473 (#5301), Total ERK1/2 (#4695), P-S6K (#9234), HA-tag (#3724), P-MEK1/2 (#9154), P-p90RSK S380 (#11989), P-FAK Y925 (#3284). Epitomics antibodies used: Phospho-FAK Y397 (#2211-1), Phospho-FAK Y576/577 (#2183-1), Total FAK1 (#2146-1), Zyxin (#3586-1). BD Transduction Labs antibodies used: Paxillin (P13520). Sigma antibodies used: Actin (A5441), Flag polyclonal (F7425). Protein Tech antibodies used: MARK1 (21552-1-AP), MARK2 (15492-1-AP). Millipore antibodies used: ZEB2 (ABT332), MARK4 (07-699). Abcam antibodies used Twist (ab50887), IRSp53 (ab15697). CLASP2 antibody was from Santa Cruz Biotechnology (sc-98440). DIXDC1 total antibody was from R&D Systems (AF5599). Phospho-DIXDC1 S592 was developed in collaboration with Antony Wood at Cell Signaling Technologies (CST, Danvers, MA).
Goodwin J.M., Svensson R.U., Lou H.J., Winslow M.M., Turk B.E, & Shaw R.J. (2014). A novel AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential. Molecular cell, 55(3), 436-450.
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2

NF-κB Pathway Antibodies and Assays

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Antibodies against p-RelA-S536 (#3033; 1:1000), RelA (#8242; 1:1000), RelB (#4922, 1:2000; IHC: #10544), c-Rel (#4727; 1:1000), NF-κB2 (#3017; 1:1000), LKB1 (#3050; 1:1000), and NUAK1 (#4458; 1:1000) were purchased from Cell Signalling Technology (Danvers, MA). Antibodies against lamin A/C (MAB3211; 1:500) and actin (A2066; 1:20000) was purchased from Millipore (Temecula, CA, USA). Antibody against tubulin (T5168; 1:20000) was purchased from Sigma. HRP-conjugated antibodies against mouse IgG (NA931; 1:10000) and rabbit IgG (NA934; 1:10000) were purchased from Cytiva. All antibodies were diluted in tris-buffered saline-Tween 20 containing 5% bovine serum albumin, with the exception of NUAK1 (tris-buffered saline-Tween 20 containing 5% nonfat milk). BAY 11-7082 was purchased from Cayman Chemical (cat. 10010266; Ann Arbor, MI, USA). ROS-Glo H2O2 Assay was purchased from Promega (cat. G8820).
Buensuceso A., Fritz J.L., Collins O., Valdés Y.R., Borrelli M.J., DiMattia G.E, & Shepherd T.G. (2022). Loss of LKB1-NUAK1 signalling enhances NF-κB activity in a spheroid model of high-grade serous ovarian cancer. Scientific Reports, 12, 3011.
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3

Quantifying NF-κB Pathway Activation

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Antibodies against p-RelA-S536 (#3033; 1:1000), RelA (#8242; 1:1000), RelB (#4922, 1:2000; IHC: #10544), c-Rel (#4727; 1:1000), NF-ΚB2 (#3017; 1:1000), LKB1 (#3050; 1:1000), and NUAK1 (#4458; 1:1000) were purchased from Cell Signalling Technology (Danvers, MA). Antibodies against lamin A/C (MAB3211; 1:500) and actin (A2066; 1:20000) was purchased from Millipore (Temecula, CA, USA).
Antibody against tubulin (T5168; 1:20000) was purchased from Sigma. HRP-conjugated antibodies against mouse IgG (NA931; 1:10000) and rabbit IgG (NA934; 1:10000) were purchased from Cytiva. All antibodies were diluted in tris-buffered saline-Tween 20 containing 5% bovine serum albumin, with the exception of NUAK1 (tris-buffered saline-Tween 20 containing 5% nonfat milk). BAY 11-7082 was purchased from Cayman Chemical (cat. 10010266; Ann Arbor, MI, USA). ROS-Glo H 2 O 2 Assay was purchased from Promega (cat. G8820).
Buensuceso A., Fritz J.L., Collins O., Valdés Y.R., DiMattia G.E., & Shepherd T.G. (2021). Loss of LKB1-NUAK1 Signalling Enhances NF-κB Activity in a Spheroid Model of High-Grade Serous Ovarian Cancer.
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