The SVs and PGs were extracted, fixed in Davidson's solution for 2 days and then stored in formalin solution (Cat no: K46046503441, Merck) prior subjected to histological processes. The tissues were dehydrated with a series of graded alcohol solutions. The dehydrated tissues were then infiltrated and embedded in a small block of paraffin wax (Paraplast, USA) before sectioning at 5 micrometer (μm) thickness by using a microtome (Cat no: 08050282, Feather). The tissue sections were then mounted on the microscope slides. This was followed by the tissue clearance process in xylene (Cat no: 1330-20-7, Systerm) and staining with hematoxylin and eosin solution. A mounting medium, dibutyl phthalate polystyrene xylene (Cat no: 100579, Merck) was used to adhere to the tissue on the slide with the coverslip. The slides were then observed under light microscope (Olympus, Japan) with ×20 and ×40.
Dibutyl phthalate polystyrene xylene
Manufactured by Merck Group
Dibutyl phthalate polystyrene xylene is a laboratory reagent used as a solvent and plasticizer. It is commonly utilized in various analytical and research applications.
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Lab products found in correlation
2 protocols using dibutyl phthalate polystyrene xylene
1
Histological Analysis of Fixed Tissue Samples
2
Histological Analysis of Olfactory Tissues
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A total of 60 olfactory tissues were fixed in aqueous Bouin's fluid for about 18 h, washed in 70% ethanol, dehydrated through graded ethanol solutions, cleared in benzene, and embedded in paraffin wax (Sigma-Aldrich, melting point 56-58 °C) for 1 h and 30 min. Transverse sections of tissue block were cut at 4-μm thickness using a rotary microtome (Weswox MT-1090A Senior). Dewaxed tissue sections were stained with Azan Trichrome (AT) (Heidenhain, 1915 ), Mallory's Triple (MT) (Mallory, 1936) (link), and Delafield's hematoxylin-eosin (HE) (Fischer et al., 2008) (link) stain. The sections were cleared with xylene and mounted with dibutylphthalate polystyrene xylene (Merck). The stained slides were observed and photographed using a light microscope (Carl Zeiss Primo Star) equipped with a Tucsen 5.0 MP camera at different magnifications.
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