Freshly isolated BM cells were suspended in PBS with 1% fetal bovine serum and stained with BV510-Sca-1 (Biolegend, San Diego, CA), APC780-c-Kit (eBioscience, San Diego, CA), PE-Cy7-CD150 (Biolegend), Alexa700-CD16/32 (eBioscience), PE-IL-7R (BD Biosciences), APC-CD41 (BD Biosciences), BV650-CD105 (Biolegend), FITC-CD71 (BD Biosciences), BV421-TER119 (Biolegend), and PerCP-Cy5.5 conjugated lineage markers including CD4, CD8, CD3, CD5, Gr-1, CD11b, CD19 and B220 (BD Biosciences). The samples were analyzed using BD LSR II SORP and Diva 7.0 software (GRU Cancer Center Flow Cytometry Facility). For competitive repopulation assays, BV421-CD45.2 (Biolegend) was used to substitute BV421-TER119. Cell sorting of HSCs and progenitor cells was performed using BD FACSAria II SORP.
Bv421 ter119
Manufactured by BioLegend
BV421-TER119 is a fluorochrome-conjugated antibody product from BioLegend. It is designed for the detection and analysis of TER-119 antigen expression on cells using flow cytometry.
Automatically generated - may contain errors
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2 protocols using bv421 ter119
1
Immunophenotyping and Cell Sorting of Murine Hematopoietic Stem Cells
2
Immunophenotyping Mouse Blood Cell Populations
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Blood was collected from mice via the retro-orbital sinus, and red blood cells were lysed before staining with the following fluorochrome-conjugated antibodies: BV650 CD45.1 (BioLegend clone A20), AlexaFluor700 CD45.2 (BioLegend clone 104), BUV496 B220 (BD Biosciences clone RA3-6B2), PerCP-Cy5.5 CD3e (BioLegend clone 145-2C11), APC-Cy7 CD11b (BioLegend clone M1/70), APC Ly6g (BioLegend clone 1A8), BV605 Ly6c (BioLegend clone HK1.4), BV421 Ter-119 (BioLegend clone TER-119), and PE-Cy7 F4/80 (Invitrogen BM8). Data were collected using a LSRII (BD Biosciences) and analyzed using FlowJo V10 (BD Biosciences). The following cell surface markers were used to isolate or phenotype the following cell types: hematopoietic stem cells (HSCs), Lin-Sca-1+ c-Kit+ Flt3-CD150+ CD48-; HSPCs, Lin-Sca-1+ c-Kit+; granulocyte/macrophage-primed multipotent progenitors (MPP G/M ), Lin-Sca-1+ c-Kit+ Flt3-CD150-CD48+; common myeloid progenitor cells (CMPs) Lin-Sca-1-c-Kit+ CD34+ FcγR-; and granulocyte-macrophage progenitors (GMPs), Lin-Sca-1-c-Kit+ CD34+ FcγR+. Data were collected using a BD FACSymphony A5 or cells were prospectively isolated using a FACSymphony S6 (BD Biosciences). All flow cytometry data were analyzed using FlowJo V10.
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