The isolation of microglia in the brain parenchyma was performed as described previously30 (link). Briefly, the mice were anesthetized and perfused with PBS buffer. The brain was extracted and the hypothalamus was dissociated. The single cell suspension was prepared using a neural dissociation kit (Miltenyi Biotech, 130-092-628, Bergisch Gladbach, North Rhine-Westphalia, Germany), and then centrifuged at 700 × g (Thermo Fisher) for 15 min in a continuous 30% Percoll gradient. Next, the cells were incubated with biotin-conjugated anti-CD11b antibody (Thermo Fisher, 13-0112-82), and CD11b positive cells were then isolated by Dynabeads Biotin Binder (Thermo Fisher, 11047).
Biotin conjugated anti cd11b antibody
Manufactured by Thermo Fisher Scientific
The Biotin-conjugated anti-CD11b antibody is a monoclonal antibody that binds to the CD11b cell surface antigen. CD11b is a marker for myeloid cells, including monocytes, macrophages, and neutrophils. The antibody is conjugated with biotin, which can be used for various detection and isolation applications.
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Lab products found in correlation
2 protocols using biotin conjugated anti cd11b antibody
1
Isolation of Brain Microglia
2
Isolation of Microglial Cells from Mouse Hippocampus
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Microglia were isolated as previously described [11 (link)]. Mice were anesthetized and hippocampi were dissociated from the brain and transferred to HBSS buffer. A neural dissociation kit (product No. 130-092-628, Miltenyi Biotec, Waltham, MA) was used to prepare a single-cell suspension, followed by a continuous 30% Percoll gradient at 700 g for 15 min. For microglia/macrophage isolation, cells were incubated with biotin-conjugated anti-CD11b antibody (eBioscience product no. 13-0112-82, Thermo Fisher Scientific, Waltham, MA), and CD11b + cells were isolated using Dynabeads Biotin Binder (Invitrogen product no. 11047, Thermo Fisher Scientific) and following manufacturer’s instruction.
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