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Anti tgf β antibody 1d11

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The Anti-TGF-β antibody (1D11.16.8) is a monoclonal antibody that specifically binds to and neutralizes the transforming growth factor-beta (TGF-β) cytokine. It is a laboratory research tool used to study the role of TGF-β signaling in various biological processes.

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Lab products found in correlation

Anti mr1 26, by BioLegend (2 mentions) Ac 6 fp, by Cayman Chemical (2 mentions) Odn1826, by InvivoGen (2 mentions) 5 op ru, by InvivoGen (1 mentions) Np klh, by Biosearch Technologies (1 mentions) Np lps, by Biosearch Technologies (1 mentions) Np klh, by Merck Group (1 mentions) Complete freund s adjuvant, by Merck Group (1 mentions) Jes5 2a5, by BioXCell (1 mentions) Imject alum, by Thermo Fisher Scientific (1 mentions)

3 protocols using anti tgf β antibody 1d11

1

Therapeutic and Prophylactic Respiratory Vaccination

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For therapeutic vaccination, infected mice were anesthetized with isoflurane, suspended vertically, and their tongues were pulled aside to expose the pharynx43 (link), and then 1 μM 5-OP-RU or Ac6-FP (Cayman Chemical) diluted in 30 μl of PBS was administrated iph into the airway once in a week for 3 weeks. Alternatively, mice were treated with combination of 200 μg each of neutralizing antibody for cytokine blockade. In prophylactic vaccination experiments, iph inoculation with 5-OP-RU or CpG (10 μg of ODN1826, InvivoGen) alone or both in 30 μl of PBS was performed on naive mice. In some experiments, mice were inoculated iph with ~150 CFU of Mtb in combination with 20 μg of isotype, anti-MR1 (26.5, BioLegend), or anti-TGF-β antibody (1D11.16.8, Bio X cell) in 30 μl of PBS on day 0, and 100 μg each of antibody was given via intraperitoneally on day 5 p.i.
Sakai S., Kauffman K.D., Oh S., Nelson C.E., Barry CE I.I.I, & Barber D.L. (2020). MAIT cell-directed therapy of Mycobacterium tuberculosis infection. Mucosal Immunology, 14(1), 199-208.
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2

Modulation of Humoral Immune Responses

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Sex- and age-matched C57BL/6J mice were intraperitoneally (i.p.) administered 200 µg NP-KLH (Biosearch Technologies, Novato, CA, USA) emulsified with an equal amount of Imject Alum (Thermo Scientific), 100 µg NP-KLH emulsified with complete Freund’s adjuvants (Sigma), or 20–100 µg NP-LPS (Biosearch Technologies). Mouse sera were collected for ELISA, and isolated splenocytes were analyzed by flow cytometry at day 7 following the immunization. pCAGGS-Il10, pCAGGS-Tgfb1, and/or pCAGGS-Tgfb3 vectors were administered i.v. 1 day before the indicated immunization. Anti-IL-10 mAb (300 µg) (JES5-2A5, Bio X Cell, West Lebanon, NH, USA) and 300 µg anti-TGF-β antibody (1D11.16.8, Bio X Cell) were administered i.p. at day −1, 2, and 5.
Komai T., Inoue M., Okamura T., Morita K., Iwasaki Y., Sumitomo S., Shoda H., Yamamoto K, & Fujio K. (2018). Transforming Growth Factor-β and Interleukin-10 Synergistically Regulate Humoral Immunity via Modulating Metabolic Signals. Frontiers in Immunology, 9, 1364.
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3

Intranasal Vaccination and Antibody Therapy

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For therapeutic vaccination, infected mice were anesthetized with isoflurane, suspended vertically, and their tongues were pulled aside to expose the pharynx43 (link), and then 1 μM 5-OP-RU or Ac6-FP (Cayman Chemical) diluted in 30 μl of PBS was administrated intrapharyngeally (iph) into the airway once in a week for 3 weeks. Alternatively, mice were treated with combination of 200 μg each of neutralizing antibody for cytokine blockade. In prophylactic vaccination experiments, iph inoculation with 5-OP-RU or CpG (10 μg of ODN1826, InvivoGen) alone or both in 30 μl of PBS was performed on naïve mice. In some experiments, mice were inoculated iph with ~150 CFU of Mtb in combination with 20 μg of isotype, anti-MR1 (26.5, BioLegend) or anti-TGF-β antibody (1D11.16.8, BioXcell) in 30 μl of PBS on day 0, and 100 μg each of antibody was given via intraperitoneally on day 5 p.i.
Sakai S., Kauffman K.D., Oh S., Nelson C.E., Barry CE I.I.I, & Barber D.L. (2020). MAIT cell-directed therapy of Mycobacterium tuberculosis infection. Mucosal Immunology, 14(1), 199-208.
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+ Expand

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