Ab66587

Manufactured by Abcam

Sourced in United Kingdom

Ab66587 is an antibody product manufactured by Abcam. It is a primary antibody used for the detection of a specific target protein in various experimental techniques.

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Lab products found in correlation

3 protocols using ab66587

Antibody Panel for Cellular Analysis

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Antibodies used for experiments included anti-GFP (1:1000; sc-9996; Santa Cruz Biotechnology, Dallas, TX, USA); anti-GST (1:5000; sc-138; Santa Cruz Biotechnology), anti-His (1:1000; 66005-1-lg; Proteintech, Rosemont, IL, USA), anti-Actin (1:10000; sc-47778; Santa Cruz Biotechnology), anti-Lamin A/C (1:10000; sc-376248; Santa Cruz Biotechnology), anti-Progerin (1:100; sc-81611; Santa Cruz Biotechnology), anti-Progerin (1:300; ab66587; Abcam, Cambridge, UK), anti-Ki67 (1:200; Ab15580; Abcam), anti-cyclin B1 (1:100; sc-594; Santa Cruz Biotechnology), and anti-p16-INK4A (1:500; 10883-1-AP; Proteintech).

Kang S.M., Yoon M.H., Ahn J., Kim J.E., Kim S.Y., Kang S.Y., Joo J., Park S., Cho J.H., Woo T.G., Oh A.Y., Chung K.J., An S.Y., Hwang T.S., Lee S.Y., Kim J.S., Ha N.C., Song G.Y, & Park B.J. (2021). Progerinin, an optimized progerin-lamin A binding inhibitor, ameliorates premature senescence phenotypes of Hutchinson-Gilford progeria syndrome. Communications Biology, 4, 5.

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Western Blot and Immunofluorescence Assay

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For western blotting: β-actin (AMB-7229; Nordic Biosite), α-tubulin (ab4074, Abcam), Histone H3 (ab1791, Abcam), lamin A/C (sc-20681; Santa Cruz), progerin (ab66587; Abcam), PAR (ab14460; Abcam), SIRT1 (04-1557; Millipore), PGC1α (NBP1-04676ss; Novus), NAMPT (A0256; ABclonal), p53 (sc-126; Santa Cruz), p53-acetyl K382 (ab75754; Abcam), Total OXPHOS (ab110413, Abcam). For immunofluorescence: TOM20 (ab56783; Abcam), dsDNA (ab27156, Abcam), LC3 (ab192890; Abcam).

Maynard S., Hall A., Galanos P., Rizza S., Yamamoto T., Gram H.H., Munk S.H., Shoaib M., Sørensen C.S., Bohr V.A., Lerdrup M., Maya-Mendoza A, & Bartek J. (2022). Lamin A/C impairments cause mitochondrial dysfunction by attenuating PGC1α and the NAMPT-NAD+ pathway. Nucleic Acids Research, 50(17), 9948-9965.

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Immunoblotting Antibody Panel for Cellular Analyses

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The antibodies used for experiments included antibodies against GFP (1:1000; sc-9996; Santa Cruz Biotechnology, Dallas, TX, USA); GST (1:5000; sc-138; Santa Cruz Biotechnology), actin (1:10,000; sc-47778; Santa Cruz Biotechnology), WRN (1:300; GTX101081; GeneTex and 1:300; sc-376182; Santa Cruz Biotechnology), lamin A/C (1:10,000; sc-376248; Santa Cruz Biotechnology), progerin (1:300; sc-81611; Santa Cruz Biotechnology and ab66587; Abcam, Cambridge, UK), Ki67 (1:400; ab15580; Abcam), H3K9me3 (1:400 and 1:2000; ab8898; Abcam), p16-INK4A (1:300; 10,883–1-AP; Proteintech, Rosemont, IL, USA), cyclin B1 (1:200; sc-594; Santa Cruz), cdc25C (1:500; #4688; Cell Signaling technology) and Rad51 (1:200; #05-530; Millipore).

Kang S.M., Yoon M.H., Lee S.J., Ahn J., Yi S.A., Nam K.H., Park S., Woo T.G., Cho J.H., Lee J., Ha N.C, & Park B.J. (2021). Human WRN is an intrinsic inhibitor of progerin, abnormal splicing product of lamin A. Scientific Reports, 11, 9122.

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