Ten whole female flies (5–7 days old) were homogenized in PBS (Teknova P0191). Protein samples were reduced and denatured in SDS-PAGE sample buffer (BioRad 161–0747) for 20 min 95°C, separated on a 4–20% polyacrylamide gel (BioRad 456–1096) and transferred to a PVDF membrane (BioRad 1704272). Extracts were immunoblotted with rabbit anti-AKT (1:1000, Cell Signaling Technologies 9272), rabbit anti-Ser505-phospho-dAKT (1:1000, Cell Signaling Technologies 4054), mouse anti-β-Tubulin (1:2500, Developmental Studies Hybridoma Bank AB_579794), anti-mouse and anti-rabbit IgG horseradish peroxidase-conjugated secondary antibodies (1:5000, Cell Signaling Technologies, 7074 and 7072, respectively).
Hybridoma bank ab 579794
Manufactured by Developmental Studies Hybridoma Bank
The Hybridoma Bank AB_579794 is a laboratory equipment designed for the production and storage of hybridoma cell lines. It provides a controlled environment for the growth and maintenance of these cells, which are essential for the development of monoclonal antibodies. The core function of this product is to facilitate the stable and efficient cultivation of hybridoma cells, enabling researchers to generate and preserve these valuable biological resources.
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Lab products found in correlation
Rabbit anti akt, by Cell Signaling Technology (3 mentions)
Polyacrylamide gel, by Bio-Rad (3 mentions)
Pvdf membrane, by Bio-Rad (3 mentions)
Sds page sample buffer, by Bio-Rad (3 mentions)
P0191, by Teknova (3 mentions)
Rabbit anti ser505 phospho dakt, by Cell Signaling Technology (3 mentions)
Anti mouse and anti rabbit igg horseradish peroxidase conjugated secondary antibodies, by Cell Signaling Technology (3 mentions)
3 protocols using hybridoma bank ab 579794
1
Quantifying Drosophila AKT Signaling
2
Quantifying Drosophila AKT Signaling
3
Quantifying Drosophila AKT Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Ten whole female flies (5–7 days old) were homogenized in PBS (Teknova P0191). Protein samples were reduced and denatured in SDS-PAGE sample buffer (BioRad 161–0747) for 20 min 95°C, separated on a 4–20% polyacrylamide gel (BioRad 456–1096) and transferred to a PVDF membrane (BioRad 1704272). Extracts were immunoblotted with rabbit anti-AKT (1:1000, Cell Signaling Technologies 9272), rabbit anti-Ser505-phospho-dAKT (1:1000, Cell Signaling Technologies 4054), mouse anti-β-Tubulin (1:2500, Developmental Studies Hybridoma Bank AB_579794), anti-mouse and anti-rabbit IgG horseradish peroxidase-conjugated secondary antibodies (1:5000, Cell Signaling Technologies, 7074 and 7072, respectively).
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