Liver cancer HepG2 cells and non-cancer WRL cells (American Type Culture Collection, Rockville, VA, USA) were cultured in RPMI– 1640 phenol red-free media (Sigma Chemicals) supplemented with 10% fetal bovine serum (Invitrogen Life Technology, Rockville, MD) and incubated at 37°C and 5% carbon dioxide. These cells were routinely subcultured at 80% confluency. Three days prior to the experiment, the cultures were switched to RPMI– 1640 phenol red-free media (Sigma Chemicals) containing 5% charcoal-dextran-treated fetal bovine serum (Hyclone, Utah, USA). Sub-confluent cell cultures were treated with various concentrations of luteolin with DMSO as the carrier solvent. The final concentration of the solvent was 0.1% v/v, and control cultures received DMSO only. The cell density in each experiment was maintained at 5 × 102 cells/mm2.
Rpmi 1640 phenol red free media
Manufactured by Merck Group
Sourced in United States
RPMI-1640 phenol red-free media is a cell culture medium formulation that does not contain the pH indicator phenol red. It is designed to support the growth and maintenance of a variety of cell types in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hepg2 cell, by American Type Culture Collection (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 media, by Merck Group (1 mentions)
Wrl68 cells, by American Type Culture Collection (1 mentions)
2 protocols using rpmi 1640 phenol red free media
1
Luteolin's Effects on Liver Cancer Cells
2
Evaluating Apigenin's Effects on WRL-68 Cells
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WRL-68 cells (American Type Culture Collection) were cultured in Roswell Park Memorial Institute (RPMI)-1640 media (Sigma Chemicals) with 10 % foetal bovine serum (Invitrogen Life Technology) and incubated at 378C and 5 % CO 2 . These cells were routinely subcultured when reaching 80 % of confluency. At 1 d before the experiment, the cultures were switched to RPMI-1640 phenol red free media (Sigma Chemicals) and 5 % charcoal -dextran treated foetal bovine serum (Hyclone). Subconfluent cell cultures were treated with various concentrations of apigenin with dimethyl sulphoxide as the carrier solvent. The final concentration of the solvent was 0•1 % v/v, and the control cultures received dimethyl sulphoxide only. The cell density in each experiment was maintained at 5 £ 10 2 cells/mm 2 .
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